In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment

In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment

In this study, we have determined the anticancer activity of doxorubicin (Dox)-loaded DNA/gold nanoparticle (AuNP) nanocarrier (Dox-DNA-AuNP) for the treatment of ovarian cancer. The anticancer effect of Dox-DNA-AuNP was evaluated in vitro using the EZ-Cytox cell viability assay on three human ovari...

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Main Authors: Chang-Seuk Lee, Tae Wan Kim, Da Eun Oh, Su Ok Bae, Jaesung Ryu, Hyejeong Kong, Hyeji Jeon, Hee Kyung Seo, Seob Jeon, Tae Hyun Kim
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Series:Cancers
Subjects:
nanomedicine
doxorubicin
gold nanoparticles
ovarian cancer
drug delivery
Online Access:https://www.mdpi.com/2072-6694/12/3/634
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spelling doaj-02151ab3d7a540a5b5c1d04c27c900432020-11-25T02:56:01ZengMDPI AGCancers2072-66942020-03-0112363410.3390/cancers12030634cancers12030634In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer TreatmentChang-Seuk Lee0Tae Wan Kim1Da Eun Oh2Su Ok Bae3Jaesung Ryu4Hyejeong Kong5Hyeji Jeon6Hee Kyung Seo7Seob Jeon8Tae Hyun Kim9Department of Chemistry, Soonchunhyang University, Asan 31538, KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, KoreaDepartment of Chemistry, Soonchunhyang University, Asan 31538, KoreaDepartment of Chemistry, Soonchunhyang University, Asan 31538, KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, KoreaDepartment of Obstetrics and Gynecology, College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, KoreaDepartment of Obstetrics and Gynecology, College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, KoreaDepartment of Obstetrics and Gynecology, College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, KoreaDepartment of Chemistry, Soonchunhyang University, Asan 31538, KoreaIn this study, we have determined the anticancer activity of doxorubicin (Dox)-loaded DNA/gold nanoparticle (AuNP) nanocarrier (Dox-DNA-AuNP) for the treatment of ovarian cancer. The anticancer effect of Dox-DNA-AuNP was evaluated in vitro using the EZ-Cytox cell viability assay on three human ovarian cancer cell lines, SK-OV-3, HEY A8, and A2780. Dox-DNA-AuNP exhibited outstanding activity with good IC<sub>50</sub> values of 4.8, 7.4, and 7.6 nM for SK-OV-3, HEY A8, and A2780, respectively. In vivo evaluation further demonstrated the superior anticancer effects of Dox-DNA-AuNP by inhibiting tumor growth compared to free Dox in an established SK-OV-3 xenograft mice model. Dox-DNA-AuNP showed about a 2.5 times higher tumor growth inhibition rate than free Dox. Furthermore, the immunohistochemical analysis of Ki67 antigen expression showed the lowest number of proliferative cells in the ovarian tumor tissue treated with Dox-DNA-AuNP. These results suggest Dox-DNA-AuNP might be a potential effective agent in ovarian cancer chemotherapy.https://www.mdpi.com/2072-6694/12/3/634nanomedicinedoxorubicingold nanoparticlesovarian cancerdrug delivery
collection DOAJ
language English
format Article
sources DOAJ
author Chang-Seuk Lee
Tae Wan Kim
Da Eun Oh
Su Ok Bae
Jaesung Ryu
Hyejeong Kong
Hyeji Jeon
Hee Kyung Seo
Seob Jeon
Tae Hyun Kim
spellingShingle Chang-Seuk Lee
Tae Wan Kim
Da Eun Oh
Su Ok Bae
Jaesung Ryu
Hyejeong Kong
Hyeji Jeon
Hee Kyung Seo
Seob Jeon
Tae Hyun Kim
In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment
Cancers
nanomedicine
doxorubicin
gold nanoparticles
ovarian cancer
drug delivery
author_facet Chang-Seuk Lee
Tae Wan Kim
Da Eun Oh
Su Ok Bae
Jaesung Ryu
Hyejeong Kong
Hyeji Jeon
Hee Kyung Seo
Seob Jeon
Tae Hyun Kim
author_sort Chang-Seuk Lee
title In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment
title_short In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment
title_full In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment
title_fullStr In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment
title_full_unstemmed In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment
title_sort in vivo and in vitro anticancer activity of doxorubicin-loaded dna-aunp nanocarrier for the ovarian cancer treatment
publisher MDPI AG
series Cancers
issn 2072-6694
publishDate 2020-03-01
description In this study, we have determined the anticancer activity of doxorubicin (Dox)-loaded DNA/gold nanoparticle (AuNP) nanocarrier (Dox-DNA-AuNP) for the treatment of ovarian cancer. The anticancer effect of Dox-DNA-AuNP was evaluated in vitro using the EZ-Cytox cell viability assay on three human ovarian cancer cell lines, SK-OV-3, HEY A8, and A2780. Dox-DNA-AuNP exhibited outstanding activity with good IC<sub>50</sub> values of 4.8, 7.4, and 7.6 nM for SK-OV-3, HEY A8, and A2780, respectively. In vivo evaluation further demonstrated the superior anticancer effects of Dox-DNA-AuNP by inhibiting tumor growth compared to free Dox in an established SK-OV-3 xenograft mice model. Dox-DNA-AuNP showed about a 2.5 times higher tumor growth inhibition rate than free Dox. Furthermore, the immunohistochemical analysis of Ki67 antigen expression showed the lowest number of proliferative cells in the ovarian tumor tissue treated with Dox-DNA-AuNP. These results suggest Dox-DNA-AuNP might be a potential effective agent in ovarian cancer chemotherapy.
topic nanomedicine
doxorubicin
gold nanoparticles
ovarian cancer
drug delivery
url https://www.mdpi.com/2072-6694/12/3/634
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