Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana

Background. Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of a...

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Main Authors: Ming Li, Zhaoxu Liang, Cuixia Di, Weikuan Fang, Kaichao Wu, Maoshan Chen, Shanshan He, Yuan Zeng, Yan Jing, Jun Liang, Fang Tan, Song Li, Tuo Chen, Guangxiu Liu, Lizhe An
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2016/2483258
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spelling doaj-022b38ef4356404280a46743987ae5c92020-11-24T23:17:53ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/24832582483258Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeanaMing Li0Zhaoxu Liang1Cuixia Di2Weikuan Fang3Kaichao Wu4Maoshan Chen5Shanshan He6Yuan Zeng7Yan Jing8Jun Liang9Fang Tan10Song Li11Tuo Chen12Guangxiu Liu13Lizhe An14Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, ChinaGuangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaDepartment of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, AustraliaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, ChinaCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, ChinaCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, ChinaBackground. Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures.http://dx.doi.org/10.1155/2016/2483258
collection DOAJ
language English
format Article
sources DOAJ
author Ming Li
Zhaoxu Liang
Cuixia Di
Weikuan Fang
Kaichao Wu
Maoshan Chen
Shanshan He
Yuan Zeng
Yan Jing
Jun Liang
Fang Tan
Song Li
Tuo Chen
Guangxiu Liu
Lizhe An
spellingShingle Ming Li
Zhaoxu Liang
Cuixia Di
Weikuan Fang
Kaichao Wu
Maoshan Chen
Shanshan He
Yuan Zeng
Yan Jing
Jun Liang
Fang Tan
Song Li
Tuo Chen
Guangxiu Liu
Lizhe An
Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
BioMed Research International
author_facet Ming Li
Zhaoxu Liang
Cuixia Di
Weikuan Fang
Kaichao Wu
Maoshan Chen
Shanshan He
Yuan Zeng
Yan Jing
Jun Liang
Fang Tan
Song Li
Tuo Chen
Guangxiu Liu
Lizhe An
author_sort Ming Li
title Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
title_short Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
title_full Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
title_fullStr Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
title_full_unstemmed Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
title_sort molecular characterization of lrb7 gene and a water channel protein tip2 in chorispora bungeana
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2016-01-01
description Background. Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures.
url http://dx.doi.org/10.1155/2016/2483258
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