Metabolic Profiling of Diabetic Cats in Remission
Background: The majority of diabetic cats in remission have abnormal glucose tolerance, and approximately one third relapse within 1 year. Greater understanding of the metabolic characteristics of diabetic cats in remission, and predictors of relapse is required to effectively monitor and manage the...
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doaj-0232fdef71d144198681a8aee5e7b1832020-11-25T03:08:09ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-05-01710.3389/fvets.2020.00218517616Metabolic Profiling of Diabetic Cats in RemissionSusan Gottlieb0Susan Gottlieb1Jacquie Rand2Jacquie Rand3Stephen T. Anderson4John Murray Morton5John Murray Morton6Daniel A. Dias7Berin A. Boughton8Ute Roessner9Ziad Ramadan10The Cat Clinic, Brisbane, QLD, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD, AustraliaAustralian Pet Welfare Foundation, Kenmore, QLD, AustraliaSchool of Biomedical Sciences, The University of Queensland, St. Lucia, QLD, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD, AustraliaJemora Pty Ltd, Geelong, VIC, AustraliaSchool of Health and Biomedical Sciences, Discipline of Laboratory Medicine, RMIT University, Bundoora, VIC, AustraliaMetabolomics Australia, School of BioSciences, The University of Melbourne, Melbourne, VIC, AustraliaMetabolomics Australia, School of BioSciences, The University of Melbourne, Melbourne, VIC, AustraliaNestlé Purina Research, St. Louis, MO, United StatesBackground: The majority of diabetic cats in remission have abnormal glucose tolerance, and approximately one third relapse within 1 year. Greater understanding of the metabolic characteristics of diabetic cats in remission, and predictors of relapse is required to effectively monitor and manage these cats.Objectives: To identify and compare differences in plasma metabolites between diabetic cats in remission and healthy control cats using a metabolomics approach. Secondly, to assess whether identified metabolites are predictors of diabetic relapse.Animals: Twenty cats in diabetic remission for a median of 101 days, and 22 healthy matched control cats.Methods: Cats were admitted to a clinic, and casual blood glucose was recorded. After a 24 h fast, blood glucose concentration was measured, then a blood sample was taken for metabolomic (GCMS and LCMS) analyses. Three hours later, a simplified intravenous glucose tolerance test (1 g glucose/kg) was performed. Cats were monitored for diabetes relapse for at least 9 months (270 days) after baseline testing.Results: Most cats in remission continued to display impaired glucose tolerance. Concentrations of 16 identified metabolites differed (P ≤ 0.05) between remission and control cats: 10 amino acids and stearic acid (all lower in remission cats), and glucose, glycine, xylitol, urea and carnitine (all higher in remission cats). Moderately close correlations were found between these 16 metabolites and variables assessing glycaemic responses (most |r| = 0.31 to 0.69). Five cats in remission relapsed during the study period. No metabolite was identified as a predictor of relapse.Conclusion and clinical importance: This study shows that cats in diabetic remission have abnormal metabolism.https://www.frontiersin.org/article/10.3389/fvets.2020.00218/fulldiabetes mellitusmetabolomicsdiabetic remissionfeline endocrinologyfeline diabetes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Susan Gottlieb Susan Gottlieb Jacquie Rand Jacquie Rand Stephen T. Anderson John Murray Morton John Murray Morton Daniel A. Dias Berin A. Boughton Ute Roessner Ziad Ramadan |
spellingShingle |
Susan Gottlieb Susan Gottlieb Jacquie Rand Jacquie Rand Stephen T. Anderson John Murray Morton John Murray Morton Daniel A. Dias Berin A. Boughton Ute Roessner Ziad Ramadan Metabolic Profiling of Diabetic Cats in Remission Frontiers in Veterinary Science diabetes mellitus metabolomics diabetic remission feline endocrinology feline diabetes |
author_facet |
Susan Gottlieb Susan Gottlieb Jacquie Rand Jacquie Rand Stephen T. Anderson John Murray Morton John Murray Morton Daniel A. Dias Berin A. Boughton Ute Roessner Ziad Ramadan |
author_sort |
Susan Gottlieb |
title |
Metabolic Profiling of Diabetic Cats in Remission |
title_short |
Metabolic Profiling of Diabetic Cats in Remission |
title_full |
Metabolic Profiling of Diabetic Cats in Remission |
title_fullStr |
Metabolic Profiling of Diabetic Cats in Remission |
title_full_unstemmed |
Metabolic Profiling of Diabetic Cats in Remission |
title_sort |
metabolic profiling of diabetic cats in remission |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2020-05-01 |
description |
Background: The majority of diabetic cats in remission have abnormal glucose tolerance, and approximately one third relapse within 1 year. Greater understanding of the metabolic characteristics of diabetic cats in remission, and predictors of relapse is required to effectively monitor and manage these cats.Objectives: To identify and compare differences in plasma metabolites between diabetic cats in remission and healthy control cats using a metabolomics approach. Secondly, to assess whether identified metabolites are predictors of diabetic relapse.Animals: Twenty cats in diabetic remission for a median of 101 days, and 22 healthy matched control cats.Methods: Cats were admitted to a clinic, and casual blood glucose was recorded. After a 24 h fast, blood glucose concentration was measured, then a blood sample was taken for metabolomic (GCMS and LCMS) analyses. Three hours later, a simplified intravenous glucose tolerance test (1 g glucose/kg) was performed. Cats were monitored for diabetes relapse for at least 9 months (270 days) after baseline testing.Results: Most cats in remission continued to display impaired glucose tolerance. Concentrations of 16 identified metabolites differed (P ≤ 0.05) between remission and control cats: 10 amino acids and stearic acid (all lower in remission cats), and glucose, glycine, xylitol, urea and carnitine (all higher in remission cats). Moderately close correlations were found between these 16 metabolites and variables assessing glycaemic responses (most |r| = 0.31 to 0.69). Five cats in remission relapsed during the study period. No metabolite was identified as a predictor of relapse.Conclusion and clinical importance: This study shows that cats in diabetic remission have abnormal metabolism. |
topic |
diabetes mellitus metabolomics diabetic remission feline endocrinology feline diabetes |
url |
https://www.frontiersin.org/article/10.3389/fvets.2020.00218/full |
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