Effect of pirfenidone on the proliferation of rat corneal stromal cells
AIM: To investigate the effects of pirfenidone(PFD)on the proliferation and transfomring growth factor-β<sub>1</sub>(TGF-β<sub>1</sub>)expression <i>in vitro</i> culture rat corneal stromal cells. <p>METHODS: Corneal stromal cells from 8 to 10wk SD rats were...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2015-02-01
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Series: | Guoji Yanke Zazhi |
Subjects: | |
Online Access: | http://ies.ijo.cn/cn_publish/2015/2/201502004.pdf |
Summary: | AIM: To investigate the effects of pirfenidone(PFD)on the proliferation and transfomring growth factor-β<sub>1</sub>(TGF-β<sub>1</sub>)expression <i>in vitro</i> culture rat corneal stromal cells. <p>METHODS: Corneal stromal cells from 8 to 10wk SD rats were isolated, cultured and treated with different concentrations of PFD 0mg/mL(control group), 0.15mg/mL(experimental group Ⅰ), 0.3mg/mL(experimental group Ⅱ), 1mg/mL(experimental group Ⅲ)for 48h. CCK-8 assay was performed to assess cell proliferation, while immunocytochemistry and Western Blot were used to detect the expression of ki-67 and TGF-β<sub>1</sub> expression, respectively. <p>RESULTS: Compared with control group, PFD significantly inhibited the proliferation in a dose-dependent manner(all <i>P</i><0.05), so was protein expression of ki-67. PFD significantly down-regulated the expression of TGF-β<sub>1</sub> in a dose-dependent manner(<i>P</i><0.05). <p>CONCLUSION: Pirfenidone can significantly inhibit the proliferation of rat corneal stromal cell by down regulating TGF-β<sub>1</sub> expression, therefore, it has potential prospect in lightening the corneal wound healing reaction. |
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ISSN: | 1672-5123 1672-5123 |