Effect of pirfenidone on the proliferation of rat corneal stromal cells

• Main Authors: Jun-Jie Chen, Gong-Fa Wu, Jun-Shan Lin, Yu-Ting Zeng, Qi-Ting Huang
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2015-02-01
• Series: Guoji Yanke Zazhi
• Subjects: pirfenidone; corneal stromal cells; TGF-β₁; proliferation
• Online Access: http://ies.ijo.cn/cn_publish/2015/2/201502004.pdf
• ISSN: 1672-5123; 1672-5123

AIM: To investigate the effects of pirfenidone(PFD)on the proliferation and transfomring growth factor-β₁(TGF-β₁)expression <i>in vitro</i> culture rat corneal stromal cells. <p>METHODS: Corneal stromal cells from 8 to 10wk SD rats were isolated, cultured and treated with different concentrations of PFD 0mg/mL(control group), 0.15mg/mL(experimental group Ⅰ), 0.3mg/mL(experimental group Ⅱ), 1mg/mL(experimental group Ⅲ)for 48h. CCK-8 assay was performed to assess cell proliferation, while immunocytochemistry and Western Blot were used to detect the expression of ki-67 and TGF-β₁ expression, respectively. <p>RESULTS: Compared with control group, PFD significantly inhibited the proliferation in a dose-dependent manner(all <i>P</i><0.05), so was protein expression of ki-67. PFD significantly down-regulated the expression of TGF-β₁ in a dose-dependent manner(<i>P</i><0.05). <p>CONCLUSION: Pirfenidone can significantly inhibit the proliferation of rat corneal stromal cell by down regulating TGF-β₁ expression, therefore, it has potential prospect in lightening the corneal wound healing reaction.