Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro
The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ul...
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doaj-023ea8ad92e8410c93e7d02a909730172020-11-25T01:09:21ZengElsevierSaudi Dental Journal1013-90522018-01-013013842Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitroFernanda Angelieri0Yuri Slusarenko da Silva1Daniel Araki Ribeiro2Department of Orthodontics, São Paulo Methodist University, São Bernardo do Campo, São Paulo, Brazil; Department of Orthodontics, Guarulhos University, BrazilDepartment of Orthodontics, São Paulo Methodist University, São Bernardo do Campo, São Paulo, BrazilDepartment of Biosciences, Federal University of São Paulo, Santos, São Paulo, Brazil; Corresponding author at: Departamento de Biociências, Universidade Federal de São Paulo – UNIFESP, Av. Ana Costa 95, Zip code: 11060-001, Santos, SP, Brazil.The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ultra Band Lok®) were tested in murine fibroblasts in vitro. For this purpose, eluates from each cement were prepared according manufactures instructions at 0, 2, 4, 8, 18, 32 and 64 days of immersion in artificial saliva at 37 °C. All orthodontic glass ionomer cements failed to induce cytotoxicity to murine fibroblasts for all periods evaluated in this study. However, Vidrion C® was able to induce genotoxicity after 64 days of exposure to eluates. Meron® also demonstrated genotoxicity as depicted by increasing DNA damage on 2nd day. Multicure® demonstrated genotoxicity on 32nd day and Ultra band Lok on 18th, 32nd days of exposure. Taken together, our results demonstrated that orthodontic cements derived from resin-modified glass ionomer composite (Multicure®) and compomer (Ultra Band Lok®) cause genetic damage in mammalian cells in vitro. Keywords: Genotoxicity, Cytotoxicity, Glass ionomer cements, Murine fibroblastshttp://www.sciencedirect.com/science/article/pii/S1013905217300743 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernanda Angelieri Yuri Slusarenko da Silva Daniel Araki Ribeiro |
spellingShingle |
Fernanda Angelieri Yuri Slusarenko da Silva Daniel Araki Ribeiro Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro Saudi Dental Journal |
author_facet |
Fernanda Angelieri Yuri Slusarenko da Silva Daniel Araki Ribeiro |
author_sort |
Fernanda Angelieri |
title |
Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro |
title_short |
Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro |
title_full |
Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro |
title_fullStr |
Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro |
title_full_unstemmed |
Genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro |
title_sort |
genotoxicity and cytotoxicity induced by eluates from orthodontic glass ionomer cements in vitro |
publisher |
Elsevier |
series |
Saudi Dental Journal |
issn |
1013-9052 |
publishDate |
2018-01-01 |
description |
The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ultra Band Lok®) were tested in murine fibroblasts in vitro. For this purpose, eluates from each cement were prepared according manufactures instructions at 0, 2, 4, 8, 18, 32 and 64 days of immersion in artificial saliva at 37 °C. All orthodontic glass ionomer cements failed to induce cytotoxicity to murine fibroblasts for all periods evaluated in this study. However, Vidrion C® was able to induce genotoxicity after 64 days of exposure to eluates. Meron® also demonstrated genotoxicity as depicted by increasing DNA damage on 2nd day. Multicure® demonstrated genotoxicity on 32nd day and Ultra band Lok on 18th, 32nd days of exposure. Taken together, our results demonstrated that orthodontic cements derived from resin-modified glass ionomer composite (Multicure®) and compomer (Ultra Band Lok®) cause genetic damage in mammalian cells in vitro. Keywords: Genotoxicity, Cytotoxicity, Glass ionomer cements, Murine fibroblasts |
url |
http://www.sciencedirect.com/science/article/pii/S1013905217300743 |
work_keys_str_mv |
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