<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections
Aim: Endodontic infections are caused by the invasion of various microorganisms into the root canal system. <i>Candida albicans</i> is a biofilm forming yeast and the most prevalent eukaryotic microorganism in endodontic infections. In this study we investigated the ability of <i>C...
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doaj-0243ff4b8f3a4d0a8718f5b1c7882d682020-11-25T00:39:42ZengMDPI AGAntibiotics2079-63822019-10-018420410.3390/antibiotics8040204antibiotics8040204<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic InfectionsOm Alkhir Alshanta0Suror Shaban1Christopher J Nile2William McLean3Gordon Ramage4Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G2 3JZ, UKGlasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G2 3JZ, UKGlasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G2 3JZ, UKGlasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G2 3JZ, UKGlasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G2 3JZ, UKAim: Endodontic infections are caused by the invasion of various microorganisms into the root canal system. <i>Candida albicans</i> is a biofilm forming yeast and the most prevalent eukaryotic microorganism in endodontic infections. In this study we investigated the ability of <i>C. albicans</i> to tolerate treatment with standard endodontic irrigants NaOCl (sodium hypochlorite), ethylenediaminetetraacetic acid (EDTA) and a combination thereof. We hypothesized that biofilm formed from a panel of clinical isolates differentially tolerate disinfectant regimens, and this may have implications for secondary endodontic infections. Methodology: Mature <i>C. albicans</i> biofilms were formed from 30 laboratory and oral clinical isolates and treated with either 3% NaOCl, 17% EDTA or a sequential treatment of 3% NaOCl followed by 17% EDTA for 5 min. Biofilms were then washed, media replenished and cells reincubated for an additional 24, 48 and 72 h at 37 °C. Regrowth was quantified using metabolic reduction, electrical impedance, biofilm biomass and microscopy at 0, 24, 48 and 72 h. Results: Microscopic analysis and viability readings revealed a significant initial killing effect by NaOCl, followed by a time dependent significant regrowth of <i>C. albicans</i>, but with inter-strain variability. In contrast to NaOCl, there was a continuous reduction in viability after EDTA treatment. Moreover, EDTA significantly inhibited regrowth after NaOCl treatment, though viable cells were still observed. Conclusions: Our results indicate that different <i>C. albicans</i> biofilm phenotypes grown in a non-complex surface topography have the potential to differentially tolerate standard endodontic irrigation protocols. This is the first study to report a strain dependent impact on efficacy of endodontic irrigants. Its suggested that within the complex topography of the root canal, a more difficult antimicrobial challenge, that existing endodontic irrigant regimens permit cells to regrow and drive secondary infections.https://www.mdpi.com/2079-6382/8/4/204biofilm<i>candida albicans</i>edtanaocltolerance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Om Alkhir Alshanta Suror Shaban Christopher J Nile William McLean Gordon Ramage |
spellingShingle |
Om Alkhir Alshanta Suror Shaban Christopher J Nile William McLean Gordon Ramage <i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections Antibiotics biofilm <i>candida albicans</i> edta naocl tolerance |
author_facet |
Om Alkhir Alshanta Suror Shaban Christopher J Nile William McLean Gordon Ramage |
author_sort |
Om Alkhir Alshanta |
title |
<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections |
title_short |
<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections |
title_full |
<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections |
title_fullStr |
<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections |
title_full_unstemmed |
<i>Candida albicans</i> Biofilm Heterogeneity and Tolerance of Clinical Isolates: Implications for Secondary Endodontic Infections |
title_sort |
<i>candida albicans</i> biofilm heterogeneity and tolerance of clinical isolates: implications for secondary endodontic infections |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2019-10-01 |
description |
Aim: Endodontic infections are caused by the invasion of various microorganisms into the root canal system. <i>Candida albicans</i> is a biofilm forming yeast and the most prevalent eukaryotic microorganism in endodontic infections. In this study we investigated the ability of <i>C. albicans</i> to tolerate treatment with standard endodontic irrigants NaOCl (sodium hypochlorite), ethylenediaminetetraacetic acid (EDTA) and a combination thereof. We hypothesized that biofilm formed from a panel of clinical isolates differentially tolerate disinfectant regimens, and this may have implications for secondary endodontic infections. Methodology: Mature <i>C. albicans</i> biofilms were formed from 30 laboratory and oral clinical isolates and treated with either 3% NaOCl, 17% EDTA or a sequential treatment of 3% NaOCl followed by 17% EDTA for 5 min. Biofilms were then washed, media replenished and cells reincubated for an additional 24, 48 and 72 h at 37 °C. Regrowth was quantified using metabolic reduction, electrical impedance, biofilm biomass and microscopy at 0, 24, 48 and 72 h. Results: Microscopic analysis and viability readings revealed a significant initial killing effect by NaOCl, followed by a time dependent significant regrowth of <i>C. albicans</i>, but with inter-strain variability. In contrast to NaOCl, there was a continuous reduction in viability after EDTA treatment. Moreover, EDTA significantly inhibited regrowth after NaOCl treatment, though viable cells were still observed. Conclusions: Our results indicate that different <i>C. albicans</i> biofilm phenotypes grown in a non-complex surface topography have the potential to differentially tolerate standard endodontic irrigation protocols. This is the first study to report a strain dependent impact on efficacy of endodontic irrigants. Its suggested that within the complex topography of the root canal, a more difficult antimicrobial challenge, that existing endodontic irrigant regimens permit cells to regrow and drive secondary infections. |
topic |
biofilm <i>candida albicans</i> edta naocl tolerance |
url |
https://www.mdpi.com/2079-6382/8/4/204 |
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