"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice "
Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by ionizing irradiation, through production of free radicals. It has been demonstrated that antioxidants such as vitamin E are able to protect cells from damage caused by free radicals. Taken together we found it reasona...
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Tehran University of Medical Sciences
2005-06-01
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doaj-02450dc4899b41fcab4229fe783c0ff92020-11-25T04:12:18ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492005-06-0142102"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " Bahman Maroufi0 Kaboudanian Sussan Ardestani1 Amina Kariminia2 Hussain Naderimanesh3 Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by ionizing irradiation, through production of free radicals. It has been demonstrated that antioxidants such as vitamin E are able to protect cells from damage caused by free radicals. Taken together we found it reasonable to make an attempt to evaluate the protective effect of vitamin E against apoptosis. The irradiated mice received 1 Gy/day gamma radiation for one day (low dose) or for three successive days (high dose, 3Gy). The splenocytes were then isolated at 6, 14 and 24 h after exposure. DNA gel electrophoresis and DNA fragmentation assay were done in addition to the evaluation of splenocytes cytology. Our results showed that Vitamin E can reduce apoptosis against low dose irradiation. However it is not able to completely block programmed cell death in high dose irradition. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/102Free RadicalsGamma Radiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bahman Maroufi Kaboudanian Sussan Ardestani Amina Kariminia Hussain Naderimanesh |
spellingShingle |
Bahman Maroufi Kaboudanian Sussan Ardestani Amina Kariminia Hussain Naderimanesh "The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " Iranian Journal of Allergy, Asthma and Immunology Free Radicals Gamma Radiation |
author_facet |
Bahman Maroufi Kaboudanian Sussan Ardestani Amina Kariminia Hussain Naderimanesh |
author_sort |
Bahman Maroufi |
title |
"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " |
title_short |
"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " |
title_full |
"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " |
title_fullStr |
"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " |
title_full_unstemmed |
"The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice " |
title_sort |
"the effect of vitamin e on splenocytes apoptosis of gamma-irradiated balb/c mice " |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Allergy, Asthma and Immunology |
issn |
1735-1502 1735-5249 |
publishDate |
2005-06-01 |
description |
Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by ionizing irradiation, through production of free radicals. It has been demonstrated that antioxidants such as vitamin E are able to protect cells from damage caused by free radicals. Taken together we found it reasonable to make an attempt to evaluate the protective effect of vitamin E against apoptosis. The irradiated mice received 1 Gy/day gamma radiation for one day (low dose) or for three successive days (high dose, 3Gy). The splenocytes were then isolated at 6, 14 and 24 h after exposure. DNA gel electrophoresis and DNA fragmentation assay were done in addition to the evaluation of splenocytes cytology.
Our results showed that Vitamin E can reduce apoptosis against low dose irradiation. However it is not able to completely block programmed cell death in high dose irradition.
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topic |
Free Radicals Gamma Radiation |
url |
https://ijaai.tums.ac.ir/index.php/ijaai/article/view/102 |
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AT bahmanmaroufi theeffectofvitamineonsplenocytesapoptosisofgammairradiatedbalbcmice AT kaboudaniansussanardestani theeffectofvitamineonsplenocytesapoptosisofgammairradiatedbalbcmice AT aminakariminia theeffectofvitamineonsplenocytesapoptosisofgammairradiatedbalbcmice AT hussainnaderimanesh theeffectofvitamineonsplenocytesapoptosisofgammairradiatedbalbcmice |
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1724415864385568768 |