The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region

The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region

A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated usin...

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Bibliographic Details
Main Authors: Lina Marcela Villa, Felipe Guhl, Daniel Zabala, Juan David Ramirez, Daniel Alfonso Urrea, Diana Carolina Hernandez, Zulma Cucunuba, Marleny Montilla, Julio Cesar Carranza, Karina Rueda, Jorge Eduardo Trujillo, Gustavo Adolfo Vallejo
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2013-11-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
spliced-leader intergenic region
Trypanosoma cruzi I
PCR
Chagas disease
domestic cycle
sylvatic cycle
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000700932&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000700932&lng=en&tlng=en

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