Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test

Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test

Elevated ground-level ozone (O<sub>3</sub>) reduced C-based defense chemicals; however, severe grazing damages were found in leaves grown in the low O<sub>3</sub> condition of a free air O<sub>3</sub>-concentration enrichment (O<sub>3</sub>-FACE) syste...

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Main Authors: Noboru Masui, Tomoki Mochizuki, Akira Tani, Hideyuki Matsuura, Evgenios Agathokleous, Toshihiro Watanabe, Takayoshi Koike
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Forests
Subjects:
atmospheric lifetime
biogenic volatile organic compounds (bvocs)
herbivorous insects
leaf beetle
olfactory response
ozone
Online Access:https://www.mdpi.com/1999-4907/11/1/58
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spelling doaj-02548d0c59a3474880cbec783acbd64f2020-11-25T01:12:56ZengMDPI AGForests1999-49072020-01-011115810.3390/f11010058f11010058Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube TestNoboru Masui0Tomoki Mochizuki1Akira Tani2Hideyuki Matsuura3Evgenios Agathokleous4Toshihiro Watanabe5Takayoshi Koike6Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, JapanSchool of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, JapanSchool of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanSchool of Applied Meteorology, Nanjing University of Information Science &amp; Technology (NUIST), Nanjing 210044, ChinaResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanElevated ground-level ozone (O<sub>3</sub>) reduced C-based defense chemicals; however, severe grazing damages were found in leaves grown in the low O<sub>3</sub> condition of a free air O<sub>3</sub>-concentration enrichment (O<sub>3</sub>-FACE) system. To explain this phenomenon, this study investigates the role of BVOCs (biogenic volatile organic compounds) as signaling compounds for insect herbivores. BVOCs act as scents for herbivore insects to locate host plants, while some BVOCs show high reactivity to O<sub>3</sub>, inducing changes in the composition of BVOCs in atmospheres with elevated O<sub>3</sub>. To assess the aforementioned phenomenon, profiles of BVOCs emitted from birch (<i>Betula platyphylla</i> var. <i>japonica</i> Hara) leaves were analyzed ex situ, and Y-tube insect preference tests were conducted in vitro to study the insect olfactory response. The assays were conducted in June and August or September, according to the life cycle of the adult alder leaf beetle <i>Agelastica coerulea</i> Baly (Coleoptera: Chrysomelidae). The Y-tube tests revealed that the leaf beetles were attracted to BVOCs, and O<sub>3</sub> per se had neither an attractant nor a repellent effect. BVOCs became less attractant when mixed with highly concentrated O<sub>3</sub> (&gt;80 ppb). About 20% of the total BVOCs emitted were highly O<sub>3</sub>-reactive compounds, such as <i>&#946;</i>-ocimene. The results suggest that BVOCs emitted from the birch leaves can be altered by elevated O<sub>3</sub>, thus potentially reducing the attractiveness of leaves to herbivorous insects searching for food.https://www.mdpi.com/1999-4907/11/1/58atmospheric lifetimebiogenic volatile organic compounds (bvocs)herbivorous insectsleaf beetleolfactory responseozone
collection DOAJ
language English
format Article
sources DOAJ
author Noboru Masui
Tomoki Mochizuki
Akira Tani
Hideyuki Matsuura
Evgenios Agathokleous
Toshihiro Watanabe
Takayoshi Koike
spellingShingle Noboru Masui
Tomoki Mochizuki
Akira Tani
Hideyuki Matsuura
Evgenios Agathokleous
Toshihiro Watanabe
Takayoshi Koike
Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test
Forests
atmospheric lifetime
biogenic volatile organic compounds (bvocs)
herbivorous insects
leaf beetle
olfactory response
ozone
author_facet Noboru Masui
Tomoki Mochizuki
Akira Tani
Hideyuki Matsuura
Evgenios Agathokleous
Toshihiro Watanabe
Takayoshi Koike
author_sort Noboru Masui
title Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test
title_short Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test
title_full Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test
title_fullStr Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test
title_full_unstemmed Does Ozone Alter the Attractiveness of Japanese White Birch Leaves to the Leaf Beetle <i>Agelastica coerulea</i> via Changes in Biogenic Volatile Organic Compounds (BVOCs): An Examination with the Y-Tube Test
title_sort does ozone alter the attractiveness of japanese white birch leaves to the leaf beetle <i>agelastica coerulea</i> via changes in biogenic volatile organic compounds (bvocs): an examination with the y-tube test
publisher MDPI AG
series Forests
issn 1999-4907
publishDate 2020-01-01
description Elevated ground-level ozone (O<sub>3</sub>) reduced C-based defense chemicals; however, severe grazing damages were found in leaves grown in the low O<sub>3</sub> condition of a free air O<sub>3</sub>-concentration enrichment (O<sub>3</sub>-FACE) system. To explain this phenomenon, this study investigates the role of BVOCs (biogenic volatile organic compounds) as signaling compounds for insect herbivores. BVOCs act as scents for herbivore insects to locate host plants, while some BVOCs show high reactivity to O<sub>3</sub>, inducing changes in the composition of BVOCs in atmospheres with elevated O<sub>3</sub>. To assess the aforementioned phenomenon, profiles of BVOCs emitted from birch (<i>Betula platyphylla</i> var. <i>japonica</i> Hara) leaves were analyzed ex situ, and Y-tube insect preference tests were conducted in vitro to study the insect olfactory response. The assays were conducted in June and August or September, according to the life cycle of the adult alder leaf beetle <i>Agelastica coerulea</i> Baly (Coleoptera: Chrysomelidae). The Y-tube tests revealed that the leaf beetles were attracted to BVOCs, and O<sub>3</sub> per se had neither an attractant nor a repellent effect. BVOCs became less attractant when mixed with highly concentrated O<sub>3</sub> (&gt;80 ppb). About 20% of the total BVOCs emitted were highly O<sub>3</sub>-reactive compounds, such as <i>&#946;</i>-ocimene. The results suggest that BVOCs emitted from the birch leaves can be altered by elevated O<sub>3</sub>, thus potentially reducing the attractiveness of leaves to herbivorous insects searching for food.
topic atmospheric lifetime
biogenic volatile organic compounds (bvocs)
herbivorous insects
leaf beetle
olfactory response
ozone
url https://www.mdpi.com/1999-4907/11/1/58
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