Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes
BackgroundThis study aimed to investigate the possibility of isolating mesenchymal stem cells (MSCs) from human thigh adipose tissue and the ability of human thigh adipose stem cells (HTASCs) to differentiate into hepatocytes.MethodsThe adipose-derived stem cells (ADSCs) were isolated from thigh adi...
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Korean Society of Plastic and Reconstructive Surgeons
2012-11-01
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doaj-0293f77073ce4fa088ef42c129ba0e982020-11-25T02:46:34ZengKorean Society of Plastic and Reconstructive SurgeonsArchives of Plastic Surgery2234-61632234-61712012-11-01396593599105Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional HepatocytesJong Hoon Lee0Kuk Han Lee1Min Ho Kim2Jun Pyo Kim3Seung Jae Lee4Jinah Yoon5Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine, Seoul, Korea.Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine, Seoul, Korea.Eulji Medi-Bio Research Institute, Eulji University, Seoul, Korea.CL Aesthetic Clinic, Seoul, Korea.CL Aesthetic Clinic, Seoul, Korea.Department of Biotechnology, Seoul Women's University, Seoul, Korea.BackgroundThis study aimed to investigate the possibility of isolating mesenchymal stem cells (MSCs) from human thigh adipose tissue and the ability of human thigh adipose stem cells (HTASCs) to differentiate into hepatocytes.MethodsThe adipose-derived stem cells (ADSCs) were isolated from thigh adipose tissue. Growth factors, cytokines, and hormones were added to the collagen coated dishes to induce the undifferentiated HTASCs to differentiate into hepatocyte-like cells. To confirm the experimental results, the expression of hepatocyte-specific markers on undifferentiated and differentiated HTASCs was analyzed using reverse transcription polymerase chain reaction and immunocytochemical staining. Differentiation efficiency was evaluated using functional tests such as periodic acid schiff (PAS) staining and detection of the albumin secretion level using enzyme-linked immunosorbent assay (ELISA).ResultsThe majority of the undifferentiated HTASCs were changed into a more polygonal shape showing tight interactions between the cells. The differentiated HTASCs up-regulated mRNA of hepatocyte markers. Immunocytochemical analysis showed that they were intensely stained with anti-albumin antibody compared with undifferentiated HTASCs. PAS staining showed that HTASCs submitted to the hepatocyte differentiation protocol were able to more specifically store glycogen than undifferentiated HTASCs, displaying a purple color in the cytoplasm of the differentiated HTASCs. ELISA analyses showed that differentiated HTASCs could secrete albumin, which is one of the hepatocyte markers.ConclusionsMSCs were islolated from human thigh adipose tissue differentiate to heapatocytes. The source of ADSCs is not only abundant abdominal adipose tissue, but also thigh adipose tissue for cell therapy in liver regeneration and tissue regeneration.http://www.e-aps.org/upload/pdf/aps-39-593.pdfThighAdipose tissueStem cellsHepatocytesCell Differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jong Hoon Lee Kuk Han Lee Min Ho Kim Jun Pyo Kim Seung Jae Lee Jinah Yoon |
spellingShingle |
Jong Hoon Lee Kuk Han Lee Min Ho Kim Jun Pyo Kim Seung Jae Lee Jinah Yoon Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes Archives of Plastic Surgery Thigh Adipose tissue Stem cells Hepatocytes Cell Differentiation |
author_facet |
Jong Hoon Lee Kuk Han Lee Min Ho Kim Jun Pyo Kim Seung Jae Lee Jinah Yoon |
author_sort |
Jong Hoon Lee |
title |
Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes |
title_short |
Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes |
title_full |
Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes |
title_fullStr |
Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes |
title_full_unstemmed |
Possibility of Undifferentiated Human Thigh Adipose Stem Cells Differentiating into Functional Hepatocytes |
title_sort |
possibility of undifferentiated human thigh adipose stem cells differentiating into functional hepatocytes |
publisher |
Korean Society of Plastic and Reconstructive Surgeons |
series |
Archives of Plastic Surgery |
issn |
2234-6163 2234-6171 |
publishDate |
2012-11-01 |
description |
BackgroundThis study aimed to investigate the possibility of isolating mesenchymal stem cells (MSCs) from human thigh adipose tissue and the ability of human thigh adipose stem cells (HTASCs) to differentiate into hepatocytes.MethodsThe adipose-derived stem cells (ADSCs) were isolated from thigh adipose tissue. Growth factors, cytokines, and hormones were added to the collagen coated dishes to induce the undifferentiated HTASCs to differentiate into hepatocyte-like cells. To confirm the experimental results, the expression of hepatocyte-specific markers on undifferentiated and differentiated HTASCs was analyzed using reverse transcription polymerase chain reaction and immunocytochemical staining. Differentiation efficiency was evaluated using functional tests such as periodic acid schiff (PAS) staining and detection of the albumin secretion level using enzyme-linked immunosorbent assay (ELISA).ResultsThe majority of the undifferentiated HTASCs were changed into a more polygonal shape showing tight interactions between the cells. The differentiated HTASCs up-regulated mRNA of hepatocyte markers. Immunocytochemical analysis showed that they were intensely stained with anti-albumin antibody compared with undifferentiated HTASCs. PAS staining showed that HTASCs submitted to the hepatocyte differentiation protocol were able to more specifically store glycogen than undifferentiated HTASCs, displaying a purple color in the cytoplasm of the differentiated HTASCs. ELISA analyses showed that differentiated HTASCs could secrete albumin, which is one of the hepatocyte markers.ConclusionsMSCs were islolated from human thigh adipose tissue differentiate to heapatocytes. The source of ADSCs is not only abundant abdominal adipose tissue, but also thigh adipose tissue for cell therapy in liver regeneration and tissue regeneration. |
topic |
Thigh Adipose tissue Stem cells Hepatocytes Cell Differentiation |
url |
http://www.e-aps.org/upload/pdf/aps-39-593.pdf |
work_keys_str_mv |
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