Rooting in vitro of zygotic embryos of Acrocomia aculeata (Jacq.) Lodd ex Mart.

• Main Authors: Carlos Fiori Fernández, Maura Isabel Díaz Lezcano, Luis Roberto González Segnana
• Format: Article
• Language: Spanish
• Published: Universidad Distrital Francisco Jose de Caldas 2016-01-01
• Series: Colombia Forestal
• Subjects: Cultivo oleaginoso; desarrollo de plántulas; enraizamiento in vitro; latencia
• Online Access: http://revistas.udistrital.edu.co/ojs/index.php/colfor/article/view/8083

Acrocomia aculeata is a potential specie for the energy industry, however, the latency in its seeds delay germination, affecting performance. The experiment was performed in the Biology Laboratory of the Faculty of Agricultural Sciences of the National University of Asunción; the objective was to establish a protocol for zygotic embryogenesis and rooting of this species. A completely randomized design was used, applying Kruskal-Wallis and Mann-Whitney test with 5% error probability, subject to verification of assumptions of distribution The treatments consisted of MS medium (Murashige and Skoog) supplemented with benzylaminopurine (BAP) of 1, 2 and 4 ppm, MS medium with 2 g/L of active carbon (AC) and simple MS mediums. 45 zygotic embryos were initially cultivated on MS medium supplemented with 2 g/L of AC and exposed to initial darkness for 20 days, then subcultured in the mentioned treatments. Root length was the measured variable, assessed in two periods of 20 days. There was no significant effects in the treatments with BAP, the CA suppressed explants oxidation; whereas the best results showed treatments 1 (MS + 1 ppm BAP) and 4 (MS + CA 2g/L) with 6 and 5 cm of root length respectively, after 40 incubation days.