TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS
The aim of this study is to follow the changes that occur, in time, at DNA level and to establish an efficient and reliable protocol for ancestral DNA extraction from bones found in archaeological sites. To test whether the protocol is efficient and capable of yielding good quality DNA, extraction w...
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"Alexandru Ioan Cuza" University of Iași
2012-06-01
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Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/963 |
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doaj-029bab5982d4414d92a1b8788f44bd092020-11-25T00:02:18Zeng"Alexandru Ioan Cuza" University of IașiAnalele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara 1582-35712248-32762012-06-011321318957TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINSAndrei Stefan0Lucian Gorgan1Radu Druica2Luminita BejenaruAlexandru Ioan Cuza University, IasiAlexandru Ioan Cuza University, IasiAlexandru Ioan Cuza University, IasiThe aim of this study is to follow the changes that occur, in time, at DNA level and to establish an efficient and reliable protocol for ancestral DNA extraction from bones found in archaeological sites. To test whether the protocol is efficient and capable of yielding good quality DNA, extraction was first performed on fresh bones. The material consists of fresh pig (Sus scrofa) and cow (Bos taurus) bones that were grounded by using a drill operating at low speed. The bone powder was then incubated in lysis buffer in the presence of proteinase K. DNA isolation and purification were done by using the phenol:chloroform protocol and DNA was precipitated with absolute ethanol stored at -20oC. The extractions were carried out once every month for a total of four extractionshttp://www.gbm.bio.uaic.ro/index.php/gbm/article/view/963 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrei Stefan Lucian Gorgan Radu Druica Luminita Bejenaru |
spellingShingle |
Andrei Stefan Lucian Gorgan Radu Druica Luminita Bejenaru TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
author_facet |
Andrei Stefan Lucian Gorgan Radu Druica Luminita Bejenaru |
author_sort |
Andrei Stefan |
title |
TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS |
title_short |
TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS |
title_full |
TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS |
title_fullStr |
TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS |
title_full_unstemmed |
TEMPORAL MODELING OF DNA DEGRADATION IN BONE REMAINS |
title_sort |
temporal modeling of dna degradation in bone remains |
publisher |
"Alexandru Ioan Cuza" University of Iași |
series |
Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
issn |
1582-3571 2248-3276 |
publishDate |
2012-06-01 |
description |
The aim of this study is to follow the changes that occur, in time, at DNA level and to establish an efficient and reliable protocol for ancestral DNA extraction from bones found in archaeological sites. To test whether the protocol is efficient and capable of yielding good quality DNA, extraction was first performed on fresh bones. The material consists of fresh pig (Sus scrofa) and cow (Bos taurus) bones that were grounded by using a drill operating at low speed. The bone powder was then incubated in lysis buffer in the presence of proteinase K. DNA isolation and purification were done by using the phenol:chloroform protocol and DNA was precipitated with absolute ethanol stored at -20oC. The extractions were carried out once every month for a total of four extractions |
url |
http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/963 |
work_keys_str_mv |
AT andreistefan temporalmodelingofdnadegradationinboneremains AT luciangorgan temporalmodelingofdnadegradationinboneremains AT radudruica temporalmodelingofdnadegradationinboneremains AT luminitabejenaru temporalmodelingofdnadegradationinboneremains |
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