When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing
Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun...
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01566/full |
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doaj-029f97e6f6564e419df9ed19363ac9092020-11-24T20:53:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01566389467When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation SequencingVladimir Lazarevic0Nadia Gaïa1Myriam Girard2Stefano Leo3Abdessalam Cherkaoui4Gesuele Renzi5Stéphane Emonet6Sharon Jamme7Etienne Ruppé8Sandrine Vijgen9Laura Rubbia-Brandt10Christian Toso11Jacques Schrenzel12Jacques Schrenzel13Genomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandDepartment of Community, Primary Care and Emergency Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandDivision of Clinical Pathology, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandDivision of Clinical Pathology, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandDepartment of Surgery, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Division of Infectious Diseases, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Department of Genetics and Laboratory Medicine, Faculty of Medicine, Geneva University Hospitals, Geneva, SwitzerlandHere, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun sequencing may be used together with other tests to strengthen the diagnosis of hepatic brucelloma.https://www.frontiersin.org/article/10.3389/fmicb.2018.01566/fullBrucellaliver abscessclinical metagenomicsmolecular diagnosis of infectious diseasesnext-generation sequencing |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Vladimir Lazarevic Nadia Gaïa Myriam Girard Stefano Leo Abdessalam Cherkaoui Gesuele Renzi Stéphane Emonet Sharon Jamme Etienne Ruppé Sandrine Vijgen Laura Rubbia-Brandt Christian Toso Jacques Schrenzel Jacques Schrenzel |
| spellingShingle |
Vladimir Lazarevic Nadia Gaïa Myriam Girard Stefano Leo Abdessalam Cherkaoui Gesuele Renzi Stéphane Emonet Sharon Jamme Etienne Ruppé Sandrine Vijgen Laura Rubbia-Brandt Christian Toso Jacques Schrenzel Jacques Schrenzel When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing Frontiers in Microbiology Brucella liver abscess clinical metagenomics molecular diagnosis of infectious diseases next-generation sequencing |
| author_facet |
Vladimir Lazarevic Nadia Gaïa Myriam Girard Stefano Leo Abdessalam Cherkaoui Gesuele Renzi Stéphane Emonet Sharon Jamme Etienne Ruppé Sandrine Vijgen Laura Rubbia-Brandt Christian Toso Jacques Schrenzel Jacques Schrenzel |
| author_sort |
Vladimir Lazarevic |
| title |
When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing |
| title_short |
When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing |
| title_full |
When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing |
| title_fullStr |
When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing |
| title_full_unstemmed |
When Bacterial Culture Fails, Metagenomics Can Help: A Case of Chronic Hepatic Brucelloma Assessed by Next-Generation Sequencing |
| title_sort |
when bacterial culture fails, metagenomics can help: a case of chronic hepatic brucelloma assessed by next-generation sequencing |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Microbiology |
| issn |
1664-302X |
| publishDate |
2018-07-01 |
| description |
Here, we sequenced DNA extracted from a necrotic hepatic lesion from a patient with suspected chronic hepatic brucelloma but negative culture results. Although most of the taxonomically classified sequencing reads corresponded to human genome sequences, our data suggest that whole-metagenome shotgun sequencing may be used together with other tests to strengthen the diagnosis of hepatic brucelloma. |
| topic |
Brucella liver abscess clinical metagenomics molecular diagnosis of infectious diseases next-generation sequencing |
| url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01566/full |
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