Fabrication of Biosensors Based on Nanostructured Conducting Polyaniline (NSPANI)

• Main Authors: Deepshikha SAINI, Ruchika CHAUHAN, Tinku BASU
• Format: Article
• Language: English
• Published: IFSA Publishing, S.L. 2011-11-01
• Series: Sensors & Transducers
• Subjects: Polyaniline nanoparticles; Enzyme biosensors; Glucose oxidase; Horseradish peroxidase; Glucose; Hydrogen peroxide; Spectroelectrochemistry
• Online Access: http://www.sensorsportal.com/HTML/DIGEST/november_2011/P_889.pdf

In this study, glucose and hydrogen peroxide (H2O2) biosensors based on nanostructured conducting polyaniline (NSPANI) (synthesized using sodiumdodecyl sulphate (SDS) as structure directing agent) were developed. Because of the large specific surface area, excellent conductivity of NSPANI, horseradish peroxidase (HRP) and glucose oxidase (GOx) could be easily immobilized with high loading and activity. In addition the small dimensions and the high surface-to-volume ratio of the NSCP allow the rapid transmission of electron and enhance current response. The linear dynamic range of optical glucose and H2O2 biosensors is 5–40 mM for glucose and 1–50 mM for H2O2, respectively where as the bulk PANI exhibits linearity between 5-20 mM/l. The miniature optical glucose biosensor also exhibits good reproducibility. The storage stability of optical glucose and H2O2 biosensors is two weeks for glucose and five days for H2O2. The high response value of NSPANI based biosensors as compared to bulk PANI based biosensor reflects higher enzymatic affinity of GOx/NSPANI and HRP/NSPANI with glucose and H2O2 due to biocompatibility, active surface area and high electron communication capability of nanobiopolymer film. In conclusion, the NSPANI based biosensors proposed herein have many advantages such as a low response time, high reproducibility, high sensitivity, stable and wide dynamic range.