Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study

Abstract Background To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD). Methods We conducted a literature research...

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Main Authors: Yewen Guan, Siting Wang, Jiaqi Wang, Dihua Meng, Huihong Wu, Qingjun Wei, Hua Jiang
Format: Article
Language:English
Published: BMC 2020-02-01
Series:Journal of Orthopaedic Surgery and Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13018-020-01588-8
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spelling doaj-02ad817e8d184ff59eb291a71a041b932020-11-25T00:30:54ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2020-02-0115111110.1186/s13018-020-01588-8Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical studyYewen Guan0Siting Wang1Jiaqi Wang2Dihua Meng3Huihong Wu4Qingjun Wei5Hua Jiang6Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Background To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD). Methods We conducted a literature research on PubMed, Embase, Web of Science, Cochrane Library, and China National Knowledge Infrastructure (CNKI) until February 28, 2019. We included all case-control studies about the association between IL-6 and IL-10 gene polymorphisms and LDD. The odds ratio (OR) and 95% confidence interval (CI) were calculated to estimate the strength of association. Statistical analysis was conducted by Review Manager (RevMan) 5.3 software. Furthermore, immunohistochemistry (IHC) and RT-PCR were performed to evaluate IL-6 and IL-10 expressions in the normal and degenerated disc. Results A total of 6 studies, involving 1456 cases and 1611 controls, were included in this meta-analysis. G alleles of rs1800795 and rs1800797 in the IL-6 gene were significantly associated with LDD (rs1800795: G vs. C, OR = 1.38, 95% CI = 1.16–1.64, P = 0.0002; rs1800797: G vs. A, OR = 1.35, 95% CI = 1.14–1.61, P = 0.0006). Begg’s funnel plot and Egger’s tests did not show any evidence of publication bias. IL-6 expression and IL-6 mRNA levels were significantly increased in the degenerated disc compared with those in the normal disc (IL-6 immunopositive cells, 73.68 ± 10.99% vs. 37.23 ± 6.42%, P < 0.001). Conclusions IL-6 gene polymorphisms (rs1800795 and rs1800797) were significantly associated with susceptibility to LDD. A high expression level of IL-6 may be an important risk factor for LDD.http://link.springer.com/article/10.1186/s13018-020-01588-8IL-6IL-10Lumbar disc diseaseMeta-analysisPolymorphisms
collection DOAJ
language English
format Article
sources DOAJ
author Yewen Guan
Siting Wang
Jiaqi Wang
Dihua Meng
Huihong Wu
Qingjun Wei
Hua Jiang
spellingShingle Yewen Guan
Siting Wang
Jiaqi Wang
Dihua Meng
Huihong Wu
Qingjun Wei
Hua Jiang
Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
Journal of Orthopaedic Surgery and Research
IL-6
IL-10
Lumbar disc disease
Meta-analysis
Polymorphisms
author_facet Yewen Guan
Siting Wang
Jiaqi Wang
Dihua Meng
Huihong Wu
Qingjun Wei
Hua Jiang
author_sort Yewen Guan
title Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
title_short Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
title_full Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
title_fullStr Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
title_full_unstemmed Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
title_sort gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
publisher BMC
series Journal of Orthopaedic Surgery and Research
issn 1749-799X
publishDate 2020-02-01
description Abstract Background To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD). Methods We conducted a literature research on PubMed, Embase, Web of Science, Cochrane Library, and China National Knowledge Infrastructure (CNKI) until February 28, 2019. We included all case-control studies about the association between IL-6 and IL-10 gene polymorphisms and LDD. The odds ratio (OR) and 95% confidence interval (CI) were calculated to estimate the strength of association. Statistical analysis was conducted by Review Manager (RevMan) 5.3 software. Furthermore, immunohistochemistry (IHC) and RT-PCR were performed to evaluate IL-6 and IL-10 expressions in the normal and degenerated disc. Results A total of 6 studies, involving 1456 cases and 1611 controls, were included in this meta-analysis. G alleles of rs1800795 and rs1800797 in the IL-6 gene were significantly associated with LDD (rs1800795: G vs. C, OR = 1.38, 95% CI = 1.16–1.64, P = 0.0002; rs1800797: G vs. A, OR = 1.35, 95% CI = 1.14–1.61, P = 0.0006). Begg’s funnel plot and Egger’s tests did not show any evidence of publication bias. IL-6 expression and IL-6 mRNA levels were significantly increased in the degenerated disc compared with those in the normal disc (IL-6 immunopositive cells, 73.68 ± 10.99% vs. 37.23 ± 6.42%, P < 0.001). Conclusions IL-6 gene polymorphisms (rs1800795 and rs1800797) were significantly associated with susceptibility to LDD. A high expression level of IL-6 may be an important risk factor for LDD.
topic IL-6
IL-10
Lumbar disc disease
Meta-analysis
Polymorphisms
url http://link.springer.com/article/10.1186/s13018-020-01588-8
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