Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study
Abstract Background To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD). Methods We conducted a literature research...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-02-01
|
Series: | Journal of Orthopaedic Surgery and Research |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13018-020-01588-8 |
id |
doaj-02ad817e8d184ff59eb291a71a041b93 |
---|---|
record_format |
Article |
spelling |
doaj-02ad817e8d184ff59eb291a71a041b932020-11-25T00:30:54ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2020-02-0115111110.1186/s13018-020-01588-8Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical studyYewen Guan0Siting Wang1Jiaqi Wang2Dihua Meng3Huihong Wu4Qingjun Wei5Hua Jiang6Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDivision of Spine Surgery, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Background To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD). Methods We conducted a literature research on PubMed, Embase, Web of Science, Cochrane Library, and China National Knowledge Infrastructure (CNKI) until February 28, 2019. We included all case-control studies about the association between IL-6 and IL-10 gene polymorphisms and LDD. The odds ratio (OR) and 95% confidence interval (CI) were calculated to estimate the strength of association. Statistical analysis was conducted by Review Manager (RevMan) 5.3 software. Furthermore, immunohistochemistry (IHC) and RT-PCR were performed to evaluate IL-6 and IL-10 expressions in the normal and degenerated disc. Results A total of 6 studies, involving 1456 cases and 1611 controls, were included in this meta-analysis. G alleles of rs1800795 and rs1800797 in the IL-6 gene were significantly associated with LDD (rs1800795: G vs. C, OR = 1.38, 95% CI = 1.16–1.64, P = 0.0002; rs1800797: G vs. A, OR = 1.35, 95% CI = 1.14–1.61, P = 0.0006). Begg’s funnel plot and Egger’s tests did not show any evidence of publication bias. IL-6 expression and IL-6 mRNA levels were significantly increased in the degenerated disc compared with those in the normal disc (IL-6 immunopositive cells, 73.68 ± 10.99% vs. 37.23 ± 6.42%, P < 0.001). Conclusions IL-6 gene polymorphisms (rs1800795 and rs1800797) were significantly associated with susceptibility to LDD. A high expression level of IL-6 may be an important risk factor for LDD.http://link.springer.com/article/10.1186/s13018-020-01588-8IL-6IL-10Lumbar disc diseaseMeta-analysisPolymorphisms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yewen Guan Siting Wang Jiaqi Wang Dihua Meng Huihong Wu Qingjun Wei Hua Jiang |
spellingShingle |
Yewen Guan Siting Wang Jiaqi Wang Dihua Meng Huihong Wu Qingjun Wei Hua Jiang Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study Journal of Orthopaedic Surgery and Research IL-6 IL-10 Lumbar disc disease Meta-analysis Polymorphisms |
author_facet |
Yewen Guan Siting Wang Jiaqi Wang Dihua Meng Huihong Wu Qingjun Wei Hua Jiang |
author_sort |
Yewen Guan |
title |
Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study |
title_short |
Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study |
title_full |
Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study |
title_fullStr |
Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study |
title_full_unstemmed |
Gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study |
title_sort |
gene polymorphisms and expression levels of interleukin-6 and interleukin-10 in lumbar disc disease: a meta-analysis and immunohistochemical study |
publisher |
BMC |
series |
Journal of Orthopaedic Surgery and Research |
issn |
1749-799X |
publishDate |
2020-02-01 |
description |
Abstract Background To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD). Methods We conducted a literature research on PubMed, Embase, Web of Science, Cochrane Library, and China National Knowledge Infrastructure (CNKI) until February 28, 2019. We included all case-control studies about the association between IL-6 and IL-10 gene polymorphisms and LDD. The odds ratio (OR) and 95% confidence interval (CI) were calculated to estimate the strength of association. Statistical analysis was conducted by Review Manager (RevMan) 5.3 software. Furthermore, immunohistochemistry (IHC) and RT-PCR were performed to evaluate IL-6 and IL-10 expressions in the normal and degenerated disc. Results A total of 6 studies, involving 1456 cases and 1611 controls, were included in this meta-analysis. G alleles of rs1800795 and rs1800797 in the IL-6 gene were significantly associated with LDD (rs1800795: G vs. C, OR = 1.38, 95% CI = 1.16–1.64, P = 0.0002; rs1800797: G vs. A, OR = 1.35, 95% CI = 1.14–1.61, P = 0.0006). Begg’s funnel plot and Egger’s tests did not show any evidence of publication bias. IL-6 expression and IL-6 mRNA levels were significantly increased in the degenerated disc compared with those in the normal disc (IL-6 immunopositive cells, 73.68 ± 10.99% vs. 37.23 ± 6.42%, P < 0.001). Conclusions IL-6 gene polymorphisms (rs1800795 and rs1800797) were significantly associated with susceptibility to LDD. A high expression level of IL-6 may be an important risk factor for LDD. |
topic |
IL-6 IL-10 Lumbar disc disease Meta-analysis Polymorphisms |
url |
http://link.springer.com/article/10.1186/s13018-020-01588-8 |
work_keys_str_mv |
AT yewenguan genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy AT sitingwang genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy AT jiaqiwang genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy AT dihuameng genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy AT huihongwu genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy AT qingjunwei genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy AT huajiang genepolymorphismsandexpressionlevelsofinterleukin6andinterleukin10inlumbardiscdiseaseametaanalysisandimmunohistochemicalstudy |
_version_ |
1725325083139899392 |