An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases
Background: Atopic dermatitis is a chronic or chronically relapsing, pruritic inflammatory skin disease. The incidence of atopic dermatitis has dramatically increased during the past three decades in industrialized countries. We attempted to develop an improved method to induce an animal model of at...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2007-01-01
|
Series: | Allergology International |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015308881 |
id |
doaj-02c9de7c75114078a3bab8192fcfea0e |
---|---|
record_format |
Article |
spelling |
doaj-02c9de7c75114078a3bab8192fcfea0e2020-11-24T21:12:09ZengElsevierAllergology International1323-89302007-01-0156440340910.2332/allergolint.O-07-486An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family KinasesYuko Kawakami0Kenji Yumoto1Toshiaki Kawakami2Division of Cell Biology, La Jolla Institute for Allergy and Immunology California, USA.Division of Cell Biology, La Jolla Institute for Allergy and Immunology California, USA.Division of Cell Biology, La Jolla Institute for Allergy and Immunology California, USA.Background: Atopic dermatitis is a chronic or chronically relapsing, pruritic inflammatory skin disease. The incidence of atopic dermatitis has dramatically increased during the past three decades in industrialized countries. We attempted to develop an improved method to induce an animal model of atopic dermatitis and to use it to evaluate the efficacy of a Tec family kinase inhibitor. Methods: We treated dermatitis-prone inbred mice, NC/Nga, by repetitive epicutaneous applications of a house dust mite allergen and staphylococcal enterotoxin B to induce atopic dermatitis-like skin lesions. Results: We established a highly efficient protocol to induce skin lesions in NC/Nga mice, which were histologically and immunologically similar to human atopic dermatitis. Similar to human patients, serum IgE levels were increased in dermatitis-induced mice. Consistent with the proposed roles of infiltrated immune cells in the pathogenesis of human atopic dermatitis, skin lesions were treatable with terreic acid, an inhibitor of Tec family kinases, as well as dexamethasone. Conclusions: We established a highly efficient, highly reproducible protocol to induce skin lesions in NC/Nga mice and successfully applied it to show the efficacy of terreic acid in treating skin lesions. This mouse model of atopic dermatitis will be useful to study the pathogenetic processes of atopic dermatitis and to evaluate the efficacy of drug candidates.http://www.sciencedirect.com/science/article/pii/S1323893015308881allergenatopic dermatitismiteTecTh2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuko Kawakami Kenji Yumoto Toshiaki Kawakami |
spellingShingle |
Yuko Kawakami Kenji Yumoto Toshiaki Kawakami An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases Allergology International allergen atopic dermatitis mite Tec Th2 |
author_facet |
Yuko Kawakami Kenji Yumoto Toshiaki Kawakami |
author_sort |
Yuko Kawakami |
title |
An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases |
title_short |
An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases |
title_full |
An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases |
title_fullStr |
An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases |
title_full_unstemmed |
An Improved Mouse Model of Atopic Dermatitis and Suppression of Skin Lesions by an Inhibitor of Tec Family Kinases |
title_sort |
improved mouse model of atopic dermatitis and suppression of skin lesions by an inhibitor of tec family kinases |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2007-01-01 |
description |
Background: Atopic dermatitis is a chronic or chronically relapsing, pruritic inflammatory skin disease. The incidence of atopic dermatitis has dramatically increased during the past three decades in industrialized countries. We attempted to develop an improved method to induce an animal model of atopic dermatitis and to use it to evaluate the efficacy of a Tec family kinase inhibitor.
Methods: We treated dermatitis-prone inbred mice, NC/Nga, by repetitive epicutaneous applications of a house dust mite allergen and staphylococcal enterotoxin B to induce atopic dermatitis-like skin lesions.
Results: We established a highly efficient protocol to induce skin lesions in NC/Nga mice, which were histologically and immunologically similar to human atopic dermatitis. Similar to human patients, serum IgE levels were increased in dermatitis-induced mice. Consistent with the proposed roles of infiltrated immune cells in the pathogenesis of human atopic dermatitis, skin lesions were treatable with terreic acid, an inhibitor of Tec family kinases, as well as dexamethasone.
Conclusions: We established a highly efficient, highly reproducible protocol to induce skin lesions in NC/Nga mice and successfully applied it to show the efficacy of terreic acid in treating skin lesions. This mouse model of atopic dermatitis will be useful to study the pathogenetic processes of atopic dermatitis and to evaluate the efficacy of drug candidates. |
topic |
allergen atopic dermatitis mite Tec Th2 |
url |
http://www.sciencedirect.com/science/article/pii/S1323893015308881 |
work_keys_str_mv |
AT yukokawakami animprovedmousemodelofatopicdermatitisandsuppressionofskinlesionsbyaninhibitoroftecfamilykinases AT kenjiyumoto animprovedmousemodelofatopicdermatitisandsuppressionofskinlesionsbyaninhibitoroftecfamilykinases AT toshiakikawakami animprovedmousemodelofatopicdermatitisandsuppressionofskinlesionsbyaninhibitoroftecfamilykinases AT yukokawakami improvedmousemodelofatopicdermatitisandsuppressionofskinlesionsbyaninhibitoroftecfamilykinases AT kenjiyumoto improvedmousemodelofatopicdermatitisandsuppressionofskinlesionsbyaninhibitoroftecfamilykinases AT toshiakikawakami improvedmousemodelofatopicdermatitisandsuppressionofskinlesionsbyaninhibitoroftecfamilykinases |
_version_ |
1716751433336881152 |