Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates
Background: The efficacy of biolarvicides may be influenced by species of mosquito, larval age and density, temperature, water quality, bacterial formulation, and others. The aim of this study was to evaluate the influence of temperature and chlorine on larvicidal activity of Bacillus thuringiens...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2019-03-01
|
Series: | Journal of Arthropod-Borne Diseases |
Subjects: | |
Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/1089 |
id |
doaj-02cc123a34f841968c42afa57c2e1168 |
---|---|
record_format |
Article |
spelling |
doaj-02cc123a34f841968c42afa57c2e11682020-11-25T03:24:05ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases 1735-71792322-22712019-03-0113110.18502/jad.v13i1.9311089Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis IsolatesAileen González-Rizo0Camilo E Castañet1Ariamys Companioni2Zulema Menéndez3Hilda Hernández4M Magdalena Rodríguez5Rene Gato6Departamento Control de Vectores, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical ¨Pedro Kourí¨, La Habana, CubaFacultad de Biología, Universidad de La Habana, La Habana, CubaDepartamento Control de Vectores, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical ¨Pedro Kourí¨, La Habana, CubaDepartamento Control de Vectores, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical ¨Pedro Kourí¨, La Habana, CubaDepartamento de Parasitología, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical ¨Pedro Kourí¨, La Habana, CubaDepartamento Control de Vectores, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical ¨Pedro Kourí¨, La Habana, CubaDepartamento Control de Vectores, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical ¨Pedro Kourí¨, La Habana, Cuba Background: The efficacy of biolarvicides may be influenced by species of mosquito, larval age and density, temperature, water quality, bacterial formulation, and others. The aim of this study was to evaluate the influence of temperature and chlorine on larvicidal activity of Bacillus thuringiensis Cuban isolates against Aedes aegypti. Methods: The influence of temperature (25, 30, 35 °C) and chlorine (2.25mg/L) on the larvicidal activity of eleven B. thuringiensis Cuban isolates (collected between 2007 and 2009) were tested under laboratory conditions following WHO protocols. Bioassay data were analyzed by Probit program. The effect of chlorine and temperature (25, 30, 35 and 40 °C) on the Cry and Cyt proteins of these isolates was determined by SDS-PAGE polyacrylamide gel electrophoresis. Results: The pathogenicity of the isolates U81, X48 was affected at 35 °C. However, A21, A51, L910, and R89 isolates increase their entomopathogen activity at 35 °C. No differences were observed in toxicity of M29, R84, R85 and R87 isolates at different temperatures. The Cry 4, Cry 10 and Cry 11 proteins were reduced in A21, X48, R85 isolates at 35 and 40 °C. The Cyt proteins were reduced at 35 and 40 °C in A21, X48, R85, and A51 isolates. In L910 and R84 isolates, the Cyt toxin was degraded only at 40 °C. In chlorinated water, the lethal concentrations 50 and 90 in A21, A51, M29, R84, U81, and X48 isolates were increase. Conclusion: A21, A51, L910, R85, and X48 isolates have a strong larvicidal activity for the treatment of Ae. aegypti breeding’s sites exposed to high temperature and chlorine. https://jad.tums.ac.ir/index.php/jad/article/view/1089MosquitoesBiological controlBacillus thuringiensisBioassaysChlorine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aileen González-Rizo Camilo E Castañet Ariamys Companioni Zulema Menéndez Hilda Hernández M Magdalena Rodríguez Rene Gato |
spellingShingle |
Aileen González-Rizo Camilo E Castañet Ariamys Companioni Zulema Menéndez Hilda Hernández M Magdalena Rodríguez Rene Gato Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates Journal of Arthropod-Borne Diseases Mosquitoes Biological control Bacillus thuringiensis Bioassays Chlorine |
author_facet |
Aileen González-Rizo Camilo E Castañet Ariamys Companioni Zulema Menéndez Hilda Hernández M Magdalena Rodríguez Rene Gato |
author_sort |
Aileen González-Rizo |
title |
Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates |
title_short |
Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates |
title_full |
Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates |
title_fullStr |
Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates |
title_full_unstemmed |
Effect of Chlorine and Temperature on Larvicidal Activity of Cuban Bacillus thuringiensis Isolates |
title_sort |
effect of chlorine and temperature on larvicidal activity of cuban bacillus thuringiensis isolates |
publisher |
Tehran University of Medical Sciences |
series |
Journal of Arthropod-Borne Diseases |
issn |
1735-7179 2322-2271 |
publishDate |
2019-03-01 |
description |
Background: The efficacy of biolarvicides may be influenced by species of mosquito, larval age and density, temperature, water quality, bacterial formulation, and others. The aim of this study was to evaluate the influence of temperature and chlorine on larvicidal activity of Bacillus thuringiensis Cuban isolates against Aedes aegypti.
Methods: The influence of temperature (25, 30, 35 °C) and chlorine (2.25mg/L) on the larvicidal activity of eleven B. thuringiensis Cuban isolates (collected between 2007 and 2009) were tested under laboratory conditions following WHO protocols. Bioassay data were analyzed by Probit program. The effect of chlorine and temperature (25, 30, 35 and 40 °C) on the Cry and Cyt proteins of these isolates was determined by SDS-PAGE polyacrylamide gel electrophoresis.
Results: The pathogenicity of the isolates U81, X48 was affected at 35 °C. However, A21, A51, L910, and R89 isolates increase their entomopathogen activity at 35 °C. No differences were observed in toxicity of M29, R84, R85 and R87 isolates at different temperatures. The Cry 4, Cry 10 and Cry 11 proteins were reduced in A21, X48, R85 isolates at 35 and 40 °C. The Cyt proteins were reduced at 35 and 40 °C in A21, X48, R85, and A51 isolates. In L910 and R84 isolates, the Cyt toxin was degraded only at 40 °C. In chlorinated water, the lethal concentrations 50 and 90 in A21, A51, M29, R84, U81, and X48 isolates were increase.
Conclusion: A21, A51, L910, R85, and X48 isolates have a strong larvicidal activity for the treatment of Ae. aegypti breeding’s sites exposed to high temperature and chlorine.
|
topic |
Mosquitoes Biological control Bacillus thuringiensis Bioassays Chlorine |
url |
https://jad.tums.ac.ir/index.php/jad/article/view/1089 |
work_keys_str_mv |
AT aileengonzalezrizo effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates AT camiloecastanet effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates AT ariamyscompanioni effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates AT zulemamenendez effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates AT hildahernandez effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates AT mmagdalenarodriguez effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates AT renegato effectofchlorineandtemperatureonlarvicidalactivityofcubanbacillusthuringiensisisolates |
_version_ |
1724603538933284864 |