Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
Premise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to...
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Online Access: | https://doi.org/10.1002/aps3.1213 |
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doaj-02de4ee8e0fa4bd1b99960ef04c60e2d2020-11-25T01:15:38ZengWileyApplications in Plant Sciences2168-04502019-08-0178n/an/a10.1002/aps3.1213Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)Jun‐Wei Ye0De‐Zhu Li1Germplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 People's Republic of ChinaGermplasm Bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 People's Republic of ChinaPremise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. Conclusions The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species.https://doi.org/10.1002/aps3.1213chloroplast microsatelliteLauraceaeLindera aggregatawuyao |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun‐Wei Ye De‐Zhu Li |
spellingShingle |
Jun‐Wei Ye De‐Zhu Li Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) Applications in Plant Sciences chloroplast microsatellite Lauraceae Lindera aggregata wuyao |
author_facet |
Jun‐Wei Ye De‐Zhu Li |
author_sort |
Jun‐Wei Ye |
title |
Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) |
title_short |
Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) |
title_full |
Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) |
title_fullStr |
Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) |
title_full_unstemmed |
Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae) |
title_sort |
development of 20 chloroplast microsatellite primers in wuyao (lindera aggregata, lauraceae) |
publisher |
Wiley |
series |
Applications in Plant Sciences |
issn |
2168-0450 |
publishDate |
2019-08-01 |
description |
Premise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. Conclusions The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species. |
topic |
chloroplast microsatellite Lauraceae Lindera aggregata wuyao |
url |
https://doi.org/10.1002/aps3.1213 |
work_keys_str_mv |
AT junweiye developmentof20chloroplastmicrosatelliteprimersinwuyaolinderaaggregatalauraceae AT dezhuli developmentof20chloroplastmicrosatelliteprimersinwuyaolinderaaggregatalauraceae |
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