Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt
Abstract Background The objective of this study was to determine the prevalence of plasmid-mediated AmpC (pAmpC) among Enterobacteriaceae isolated from humans and from retail meat in Egypt. Methods Enterobacteriaceae were isolated from patients with suspected bloodstream infection, human fecal sampl...
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doaj-02e6d7823e2e4e8090cad88d12c849222020-11-25T01:10:12ZengBMCAntimicrobial Resistance and Infection Control2047-29942019-02-01811810.1186/s13756-019-0494-6Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, EgyptKatrijn L. Rensing0H. M. Abdallah1Alex Koek2Gamal A. Elmowalid3Christina M. J. E. Vandenbroucke-Grauls4Nashwan al Naiemi5Karin van Dijk6Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Microbiology and Infection Control, Amsterdam Immunity and Infection InstituteDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityAmsterdam UMC, Vrije Universiteit Amsterdam, Medical Microbiology and Infection Control, Amsterdam Immunity and Infection InstituteDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityAmsterdam UMC, Vrije Universiteit Amsterdam, Medical Microbiology and Infection Control, Amsterdam Immunity and Infection InstituteLaboratory for Medical Microbiology and Public HealthAmsterdam UMC, Vrije Universiteit Amsterdam, Medical Microbiology and Infection Control, Amsterdam Immunity and Infection InstituteAbstract Background The objective of this study was to determine the prevalence of plasmid-mediated AmpC (pAmpC) among Enterobacteriaceae isolated from humans and from retail meat in Egypt. Methods Enterobacteriaceae were isolated from patients with suspected bloodstream infection, human fecal samples, retail chicken meat samples and retail sheep meat samples. All group I Enterobacteriaceae were analyzed for presence of pAmpC genes by PCR. Antibiotic susceptibility testing was performed in all pAmpC positive isolates, followed by phenotypic and genotypic ESBL and carbapenemase testing on indication. Results The prevalence of pAmpC among group I Enterobacteriaceae isolated from 225 patients with bloodstream infection was 5.6% [95%CI 2.2–13.4]. Among 100 patients with community-onset gastroenteritis the prevalence in fecal samples was 4.8% [95%CI 2.1–10.7]. The prevalence among 112 chicken carcasses and 100 sheep meat samples was 2.4% [95%CI 0.7–8.4] and 1.1% [95%CI 0.2–5.7], respectively. In half of the AmpC positive isolates we detected an ESBL gene and 2 isolates harbored a carbapenemase gene. In five isolates there was resistance to at least three important alternative antibiotic drugs. Conclusions We consider the prevalence of pAmpC in Egypt, as found in our study, moderately low. To follow future trends in prevalence of pAmpC worldwide, a standardized screening algorithm for the detection of pAmpC is needed.http://link.springer.com/article/10.1186/s13756-019-0494-6Plasmid-mediated AmpCGroup I EnterobacteriaceaePrevalenceEgypt |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katrijn L. Rensing H. M. Abdallah Alex Koek Gamal A. Elmowalid Christina M. J. E. Vandenbroucke-Grauls Nashwan al Naiemi Karin van Dijk |
spellingShingle |
Katrijn L. Rensing H. M. Abdallah Alex Koek Gamal A. Elmowalid Christina M. J. E. Vandenbroucke-Grauls Nashwan al Naiemi Karin van Dijk Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt Antimicrobial Resistance and Infection Control Plasmid-mediated AmpC Group I Enterobacteriaceae Prevalence Egypt |
author_facet |
Katrijn L. Rensing H. M. Abdallah Alex Koek Gamal A. Elmowalid Christina M. J. E. Vandenbroucke-Grauls Nashwan al Naiemi Karin van Dijk |
author_sort |
Katrijn L. Rensing |
title |
Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt |
title_short |
Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt |
title_full |
Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt |
title_fullStr |
Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt |
title_full_unstemmed |
Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt |
title_sort |
prevalence of plasmid-mediated ampc in enterobacteriaceae isolated from humans and from retail meat in zagazig, egypt |
publisher |
BMC |
series |
Antimicrobial Resistance and Infection Control |
issn |
2047-2994 |
publishDate |
2019-02-01 |
description |
Abstract Background The objective of this study was to determine the prevalence of plasmid-mediated AmpC (pAmpC) among Enterobacteriaceae isolated from humans and from retail meat in Egypt. Methods Enterobacteriaceae were isolated from patients with suspected bloodstream infection, human fecal samples, retail chicken meat samples and retail sheep meat samples. All group I Enterobacteriaceae were analyzed for presence of pAmpC genes by PCR. Antibiotic susceptibility testing was performed in all pAmpC positive isolates, followed by phenotypic and genotypic ESBL and carbapenemase testing on indication. Results The prevalence of pAmpC among group I Enterobacteriaceae isolated from 225 patients with bloodstream infection was 5.6% [95%CI 2.2–13.4]. Among 100 patients with community-onset gastroenteritis the prevalence in fecal samples was 4.8% [95%CI 2.1–10.7]. The prevalence among 112 chicken carcasses and 100 sheep meat samples was 2.4% [95%CI 0.7–8.4] and 1.1% [95%CI 0.2–5.7], respectively. In half of the AmpC positive isolates we detected an ESBL gene and 2 isolates harbored a carbapenemase gene. In five isolates there was resistance to at least three important alternative antibiotic drugs. Conclusions We consider the prevalence of pAmpC in Egypt, as found in our study, moderately low. To follow future trends in prevalence of pAmpC worldwide, a standardized screening algorithm for the detection of pAmpC is needed. |
topic |
Plasmid-mediated AmpC Group I Enterobacteriaceae Prevalence Egypt |
url |
http://link.springer.com/article/10.1186/s13756-019-0494-6 |
work_keys_str_mv |
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