Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone
Abstract Duckweed species have a great potential to develop into fast-growing crops for water remediation and bioenergy production. Seed production and utilization of hybrid vigour are essential steps in this process. However, even in the extensively-studied duckweed species, Lemna gibba, flower pri...
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doaj-030a1bcbf9b94d5381d185ae9da5972d2020-12-08T00:58:08ZengNature Publishing GroupScientific Reports2045-23222017-06-017111310.1038/s41598-017-03240-8Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile cloneLili Fu0Meng Huang1Bingying Han2Xuepiao Sun3K. Sowjanya Sree4Klaus-J. Appenroth5Jiaming Zhang6Institute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crops Biology and Genetic Resources; Hainan Bioenergy Center, CATASInstitute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crops Biology and Genetic Resources; Hainan Bioenergy Center, CATASInstitute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crops Biology and Genetic Resources; Hainan Bioenergy Center, CATASInstitute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crops Biology and Genetic Resources; Hainan Bioenergy Center, CATASDepartment of Environmental Science, Central University of Kerala, RSTCInstitute of Plant Physiology, University of JenaInstitute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crops Biology and Genetic Resources; Hainan Bioenergy Center, CATASAbstract Duckweed species have a great potential to develop into fast-growing crops for water remediation and bioenergy production. Seed production and utilization of hybrid vigour are essential steps in this process. However, even in the extensively-studied duckweed species, Lemna gibba, flower primordia were often aborted prior to maturation. Salicylic acid (SA) and agar solidification of the medium promoted flower maturation and resulted in high flowering rates in L. gibba 7741 and 5504. Artificial cross-pollination between individuals of L. gibba 7741 yielded seeds at high frequencies unlike that in L. gibba 5504. In contrast to clone 7741, the anthers of 5504 did not dehisce upon maturation, its artificially released pollen grains had pineapple-like exine with tilted spines. These pollens were not stained by 2,5-diphenylmonotetrazoliumbromide (MTT) and failed to germinate. Therefore, clone 5504 is male sterile and has potential application with respect to hybrid vigour. Moreover, pollination of flowers of 5504 with 7741 pollen grains resulted in intraspecific hybrid seeds, which was confirmed by inter-simple sequence repeat (ISSR) markers. These hybrid seeds germinated at a high frequency, forming new clones.https://doi.org/10.1038/s41598-017-03240-8 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lili Fu Meng Huang Bingying Han Xuepiao Sun K. Sowjanya Sree Klaus-J. Appenroth Jiaming Zhang |
spellingShingle |
Lili Fu Meng Huang Bingying Han Xuepiao Sun K. Sowjanya Sree Klaus-J. Appenroth Jiaming Zhang Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone Scientific Reports |
author_facet |
Lili Fu Meng Huang Bingying Han Xuepiao Sun K. Sowjanya Sree Klaus-J. Appenroth Jiaming Zhang |
author_sort |
Lili Fu |
title |
Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone |
title_short |
Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone |
title_full |
Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone |
title_fullStr |
Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone |
title_full_unstemmed |
Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone |
title_sort |
flower induction, microscope-aided cross-pollination, and seed production in the duckweed lemna gibba with discovery of a male-sterile clone |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Abstract Duckweed species have a great potential to develop into fast-growing crops for water remediation and bioenergy production. Seed production and utilization of hybrid vigour are essential steps in this process. However, even in the extensively-studied duckweed species, Lemna gibba, flower primordia were often aborted prior to maturation. Salicylic acid (SA) and agar solidification of the medium promoted flower maturation and resulted in high flowering rates in L. gibba 7741 and 5504. Artificial cross-pollination between individuals of L. gibba 7741 yielded seeds at high frequencies unlike that in L. gibba 5504. In contrast to clone 7741, the anthers of 5504 did not dehisce upon maturation, its artificially released pollen grains had pineapple-like exine with tilted spines. These pollens were not stained by 2,5-diphenylmonotetrazoliumbromide (MTT) and failed to germinate. Therefore, clone 5504 is male sterile and has potential application with respect to hybrid vigour. Moreover, pollination of flowers of 5504 with 7741 pollen grains resulted in intraspecific hybrid seeds, which was confirmed by inter-simple sequence repeat (ISSR) markers. These hybrid seeds germinated at a high frequency, forming new clones. |
url |
https://doi.org/10.1038/s41598-017-03240-8 |
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