Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection

Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection

Background: Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub...

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Main Authors: Wan Beom Park, Leo L.M. Poon, Su-Jin Choi, Pyoeng Gyun Choe, Kyoung-Ho Song, Ji Hwan Bang, Eu Suk Kim, Hong Bin Kim, Sang Won Park, Nam Joong Kim, Malik Peiris, Myoung-don Oh
Format: Article
Language:English
Published: Elsevier 2018-07-01
Series:International Journal of Infectious Diseases
Online Access:http://www.sciencedirect.com/science/article/pii/S1201971218344114
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spelling doaj-03586f6e252e417cbe557f9ad76419de2020-11-24T21:16:53ZengElsevierInternational Journal of Infectious Diseases1201-97122018-07-0172810Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infectionWan Beom Park0Leo L.M. Poon1Su-Jin Choi2Pyoeng Gyun Choe3Kyoung-Ho Song4Ji Hwan Bang5Eu Suk Kim6Hong Bin Kim7Sang Won Park8Nam Joong Kim9Malik Peiris10Myoung-don Oh11Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea; Laboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South KoreaSchool of Public Health, The University of Hong Kong, Pokfulam, Hong Kong, ChinaLaboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea; Laboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South KoreaSchool of Public Health, The University of Hong Kong, Pokfulam, Hong Kong, China; Corresponding author.Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea; Laboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South Korea; Corresponding author at: Department of Internal Medicine, Seoul National University College of Medicine, 103 Daehak-ro, Jongro-gu, Seoul, 03080, South Korea.Background: Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens. Methods: Upper and lower respiratory samples were obtained from 17 MERS-CoV-infected patients. MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR. Results: In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA. In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate. Conclusions: These findings suggest that MERS-CoV does not replicate well in the upper respiratory tract. Keywords: Coronavirus infections, RNA, Messenger, Respiratory systemhttp://www.sciencedirect.com/science/article/pii/S1201971218344114
collection DOAJ
language English
format Article
sources DOAJ
author Wan Beom Park
Leo L.M. Poon
Su-Jin Choi
Pyoeng Gyun Choe
Kyoung-Ho Song
Ji Hwan Bang
Eu Suk Kim
Hong Bin Kim
Sang Won Park
Nam Joong Kim
Malik Peiris
Myoung-don Oh
spellingShingle Wan Beom Park
Leo L.M. Poon
Su-Jin Choi
Pyoeng Gyun Choe
Kyoung-Ho Song
Ji Hwan Bang
Eu Suk Kim
Hong Bin Kim
Sang Won Park
Nam Joong Kim
Malik Peiris
Myoung-don Oh
Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
International Journal of Infectious Diseases
author_facet Wan Beom Park
Leo L.M. Poon
Su-Jin Choi
Pyoeng Gyun Choe
Kyoung-Ho Song
Ji Hwan Bang
Eu Suk Kim
Hong Bin Kim
Sang Won Park
Nam Joong Kim
Malik Peiris
Myoung-don Oh
author_sort Wan Beom Park
title Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
title_short Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
title_full Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
title_fullStr Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
title_full_unstemmed Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection
title_sort replicative virus shedding in the respiratory tract of patients with middle east respiratory syndrome coronavirus infection
publisher Elsevier
series International Journal of Infectious Diseases
issn 1201-9712
publishDate 2018-07-01
description Background: Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens. Methods: Upper and lower respiratory samples were obtained from 17 MERS-CoV-infected patients. MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR. Results: In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA. In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate. Conclusions: These findings suggest that MERS-CoV does not replicate well in the upper respiratory tract. Keywords: Coronavirus infections, RNA, Messenger, Respiratory system
url http://www.sciencedirect.com/science/article/pii/S1201971218344114
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