Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen
Silk fibroin (SF), which offers the benefits of biosafety, biocompatibility, and mechanical strength, has potential for use as a good biomedical material, especially in the tissue engineering field. This study investigated the use of SF biomaterials as a wound dressing compared to commercially avail...
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Online Access: | https://www.mdpi.com/1420-3049/25/8/1939 |
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doaj-035c7be6916a4b5b80b6c65e02ac2bab2020-11-25T03:01:48ZengMDPI AGMolecules1420-30492020-04-01251939193910.3390/molecules25081939Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on CollagenTomoko Hashimoto0Katsura Kojima1Yasushi Tamada2Silk Materials Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, JapanSilk Materials Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, JapanSilk Materials Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, JapanSilk fibroin (SF), which offers the benefits of biosafety, biocompatibility, and mechanical strength, has potential for use as a good biomedical material, especially in the tissue engineering field. This study investigated the use of SF biomaterials as a wound dressing compared to commercially available collagen materials. After human fibroblasts (WI-38) were cultured on both films and sponges, their cell motilities and gene expressions related to wound repair and tissue reconstruction were evaluated. Compared to the collagen film (Col film), the SF film induced higher cell motility; higher expressions of genes were observed on the SF film. Extracellular matrix production-related genes were up-regulated in WI-38 fibroblasts cultured on the SF sponges. These results suggest that SF-based biomaterials can accelerate wound healing and tissue reconstruction. They can be useful biomaterials for functional wound dressings.https://www.mdpi.com/1420-3049/25/8/1939dermissilk fibrointissue engineeringwound healing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomoko Hashimoto Katsura Kojima Yasushi Tamada |
spellingShingle |
Tomoko Hashimoto Katsura Kojima Yasushi Tamada Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen Molecules dermis silk fibroin tissue engineering wound healing |
author_facet |
Tomoko Hashimoto Katsura Kojima Yasushi Tamada |
author_sort |
Tomoko Hashimoto |
title |
Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen |
title_short |
Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen |
title_full |
Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen |
title_fullStr |
Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen |
title_full_unstemmed |
Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen |
title_sort |
higher gene expression related to wound healing by fibroblasts on silk fibroin biomaterial than on collagen |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-04-01 |
description |
Silk fibroin (SF), which offers the benefits of biosafety, biocompatibility, and mechanical strength, has potential for use as a good biomedical material, especially in the tissue engineering field. This study investigated the use of SF biomaterials as a wound dressing compared to commercially available collagen materials. After human fibroblasts (WI-38) were cultured on both films and sponges, their cell motilities and gene expressions related to wound repair and tissue reconstruction were evaluated. Compared to the collagen film (Col film), the SF film induced higher cell motility; higher expressions of genes were observed on the SF film. Extracellular matrix production-related genes were up-regulated in WI-38 fibroblasts cultured on the SF sponges. These results suggest that SF-based biomaterials can accelerate wound healing and tissue reconstruction. They can be useful biomaterials for functional wound dressings. |
topic |
dermis silk fibroin tissue engineering wound healing |
url |
https://www.mdpi.com/1420-3049/25/8/1939 |
work_keys_str_mv |
AT tomokohashimoto highergeneexpressionrelatedtowoundhealingbyfibroblastsonsilkfibroinbiomaterialthanoncollagen AT katsurakojima highergeneexpressionrelatedtowoundhealingbyfibroblastsonsilkfibroinbiomaterialthanoncollagen AT yasushitamada highergeneexpressionrelatedtowoundhealingbyfibroblastsonsilkfibroinbiomaterialthanoncollagen |
_version_ |
1724691904911638528 |