Biosurfactant Production and its Role in Candida albicans Biofilm Inhibition
Biosurfactants are surface active compounds, which may be of microbial, animal or plant origin. They are typically less toxic and less persistent than the synthetically derived surfactants. The current study intended to analyze the biosurfactants production and its antagonistic activity against Ca...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2020-06-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/biosurfactant-production-and-its-role-in-candida-albicans-biofilm-inhibition/ |
| Summary: | Biosurfactants are surface active compounds, which may be of microbial, animal or plant origin. They
are typically less toxic and less persistent than the synthetically derived surfactants. The current study
intended to analyze the biosurfactants production and its antagonistic activity against Candida albicans
biofilm formation. Isolation of biosurfactant producing organism was carried out using swab sample
of human vagina and from oil contaminated soil samples. Isolates were screened for biosurfactant
production by using oil spread assay and the organisms showing higher activity were selected. The
Emulsification assay was done and the E24 was found to be 20.83% for cell free extract of growth
medium of isolate B1.The selected isolates were further studied for yield of biosurfactant produced
by cultivation in MRS broth and extraction by chloroform and methanol (3:1) extraction. The yield
of biosurfactant for isolate B1was found to be4.55gl-1.Theextracted biosurfactant was separated
by TLC and identified to be a lipopeptide by FTIR spectroscopy. The isolate with maximum yield of
biosurfactant was identified as Lactobacillus fermentum using VITEK II Compact System for microbial
identification system. The percentage biofilm inhibition activity of the biosurfactant was studied by
CFU assay followed by adhesion assay and by pre-coating experiment. On the basis of above studies,
it concludes that use of biosurfactant producing organism can be effective weapon against colonizing
opportunistic C. albicans and can be applied in medical devices for inhibition of biofilms formation.
Microbial adhesion also decreased from 85% to 11% with78.125 to 2500 µg/ml of biosurfactant. The
lipopeptide extracted from isolated isolate B1 also showed powerful penetration capacity in the biofilm
and killed 91% C. albicans as seen by CFU assay and a highest inhibition at 2500µg/ml and 1250µg/ml
concentration as studied by pre-coating experiment. |
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| ISSN: | 0973-7510 2581-690X |