Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures
This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was e...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-07-01
|
Series: | Toxins |
Subjects: | |
Online Access: | http://www.mdpi.com/2072-6651/10/7/291 |
id |
doaj-0389cfb2800a4fdfb4aecc31e505a185 |
---|---|
record_format |
Article |
spelling |
doaj-0389cfb2800a4fdfb4aecc31e505a1852020-11-25T01:41:02ZengMDPI AGToxins2072-66512018-07-0110729110.3390/toxins10070291toxins10070291Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold NanostructuresAli Ghanim Al-Rubaye0Alexei Nabok1Gaelle Catanante2Jean-Louis Marty3Eszter Takács4András Székács5Materials and Engineering Research Institute, Sheffield Hallam University, Sheffield S1 1WB, UKMaterials and Engineering Research Institute, Sheffield Hallam University, Sheffield S1 1WB, UKDepartment of Biochemistry and Molecular Biology, University of Perpignan, 66100 Perpignan, FranceDepartment of Biochemistry and Molecular Biology, University of Perpignan, 66100 Perpignan, FranceAgro-Environmental Research Institute, NARIC, 1011-1239 Budapest, HungaryAgro-Environmental Research Institute, NARIC, 1011-1239 Budapest, HungaryThis work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10−8–10−7 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers’ DNA chain length on their binding characteristics was analyzed.http://www.mdpi.com/2072-6651/10/7/291optical label-free biosensormycotoxinsaptamerLSPRgold nanostructurestotal internal reflection ellipsometery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali Ghanim Al-Rubaye Alexei Nabok Gaelle Catanante Jean-Louis Marty Eszter Takács András Székács |
spellingShingle |
Ali Ghanim Al-Rubaye Alexei Nabok Gaelle Catanante Jean-Louis Marty Eszter Takács András Székács Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures Toxins optical label-free biosensor mycotoxins aptamer LSPR gold nanostructures total internal reflection ellipsometery |
author_facet |
Ali Ghanim Al-Rubaye Alexei Nabok Gaelle Catanante Jean-Louis Marty Eszter Takács András Székács |
author_sort |
Ali Ghanim Al-Rubaye |
title |
Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures |
title_short |
Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures |
title_full |
Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures |
title_fullStr |
Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures |
title_full_unstemmed |
Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures |
title_sort |
label-free optical detection of mycotoxins using specific aptamers immobilized on gold nanostructures |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2018-07-01 |
description |
This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10−8–10−7 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers’ DNA chain length on their binding characteristics was analyzed. |
topic |
optical label-free biosensor mycotoxins aptamer LSPR gold nanostructures total internal reflection ellipsometery |
url |
http://www.mdpi.com/2072-6651/10/7/291 |
work_keys_str_mv |
AT alighanimalrubaye labelfreeopticaldetectionofmycotoxinsusingspecificaptamersimmobilizedongoldnanostructures AT alexeinabok labelfreeopticaldetectionofmycotoxinsusingspecificaptamersimmobilizedongoldnanostructures AT gaellecatanante labelfreeopticaldetectionofmycotoxinsusingspecificaptamersimmobilizedongoldnanostructures AT jeanlouismarty labelfreeopticaldetectionofmycotoxinsusingspecificaptamersimmobilizedongoldnanostructures AT esztertakacs labelfreeopticaldetectionofmycotoxinsusingspecificaptamersimmobilizedongoldnanostructures AT andrasszekacs labelfreeopticaldetectionofmycotoxinsusingspecificaptamersimmobilizedongoldnanostructures |
_version_ |
1725042936805064704 |