Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers

Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers

Summary: Liquid biopsies based on cell-free DNA (cfDNA) or exosomes provide a noninvasive approach to monitor human health and disease but have not been utilized for astronauts. Here, we profile cfDNA characteristics, including fragment size, cellular deconvolution, and nucleosome positioning, in an...

Full description

Bibliographic Details
Main Authors:	Daniela Bezdan, Kirill Grigorev, Cem Meydan, Fanny A. Pelissier Vatter, Michele Cioffi, Varsha Rao, Matthew MacKay, Kiichi Nakahira, Philip Burnham, Ebrahim Afshinnekoo, Craig Westover, Daniel Butler, Chris Mozsary, Timothy Donahoe, Jonathan Foox, Tejaswini Mishra, Serena Lucotti, Brinda K. Rana, Ari M. Melnick, Haiying Zhang, Irina Matei, David Kelsen, Kenneth Yu, David C. Lyden, Lynn Taylor, Susan M. Bailey, Michael P. Snyder, Francine E. Garrett-Bakelman, Stephan Ossowski, Iwijn De Vlaminck, Christopher E. Mason
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	iScience
Subjects:	Space Medicine Omics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004220310415

Similar Items

Microfluidic Technologies for cfDNA Isolation and Analysis
by: Zheyun Xu, et al.
Published: (2019-10-01)

cfDNA Sequencing: Technological Approaches and Bioinformatic Issues
by: Elodie Bohers, et al.
Published: (2021-06-01)

Identification of osimertinib-resistant EGFR L792 mutations by cfDNA sequencing: oncogenic activity assessment and prevalence in large cfDNA cohort
by: Stephen R. Fairclough, et al.
Published: (2019-10-01)

Comparison of the source and prognostic utility of cfDNA in trauma and sepsis
by: Nicholas L. Jackson Chornenki, et al.
Published: (2019-05-01)

Optimization of Preanalytical Variables for cfDNA Processing and Detection of ctDNA in Archival Plasma Samples
by: Marijana Nesic, et al.
Published: (2021-01-01)

Extracellular Vesicle-Derived DNA vs. CfDNA as a Biomarker for the Detection of Colon Cancer
by: Kavita Thakur, et al.
Published: (2021-07-01)

Detection of LINE-1 hypomethylation in cfDNA of Esophageal Adenocarcinoma Patients
by: Elisa Boldrin, et al.
Published: (2020-02-01)

Dynamics of Specific cfDNA Fragments in the Plasma of Full Marathon Participants
by: Takehito Sugasawa, et al.
Published: (2021-04-01)

cfDNA Changes in Maximal Exercises as a Sport Adaptation Predictor
by: Kinga Humińska-Lisowska, et al.
Published: (2021-08-01)

Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients
by: Elmo W. I. Neuberger, et al.
Published: (2021-06-01)

Beyond the Blood: CSF-Derived cfDNA for Diagnosis and Characterization of CNS Tumors
by: Abbye E. McEwen, et al.
Published: (2020-02-01)

Performance of Streck cfDNA Blood Collection Tubes for Liquid Biopsy Testing.
by: Inga Medina Diaz, et al.
Published: (2016-01-01)

Quantification of circulating cell-free DNA (cfDNA) in urine using a newborn piglet model of asphyxia.
by: Polona Rajar, et al.
Published: (2019-01-01)

Temporal patterns of circulating cell-free DNA (cfDNA) in a newborn piglet model of perinatal asphyxia.
by: Sophia Manueldas, et al.
Published: (2018-01-01)

Plasma cell-free DNA (cfDNA) as a predictive and prognostic marker in patients with metastatic breast cancer
by: Daniel Fernandez-Garcia, et al.
Published: (2019-12-01)

Plasma cfDNA as a Potential Biomarker to Evaluate the Efficacy of Chemotherapy in Gastric Cancer
by: Zhong Y, et al.
Published: (2020-05-01)

The Potential Use of Dynamics Changes of ctDNA and cfDNA in the Perioperative Period to Predict the Recurrence Risk in Early NSCLC
by: Xiaolong Zhao, et al.
Published: (2021-07-01)

Detection Cell-Free DNA (cfDNA) Using Nested-PCR as a Diagnosis Tool for Human Fascioliasis Infection
by: Mojgan ARYAEIPOUR, et al.
Published: (2020-06-01)

cfDNA testing for monitoring response to EGFR tyrosine kinase inhibitors: Time for clinical implementation?
by: Nicola Normanno, et al.
Published: (2020-07-01)

Genomic Profiling of Uterine Aspirates and cfDNA as an Integrative Liquid Biopsy Strategy in Endometrial Cancer
by: Carlos Casas-Arozamena, et al.
Published: (2020-02-01)

Utility of cfDNA Fragmentation Patterns in Designing the Liquid Biopsy Profiling Panels to Improve Their Sensitivity
by: Maxim Ivanov, et al.
Published: (2019-03-01)

Monitoring dynamic cytotoxic chemotherapy response in castration-resistant prostate cancer using plasma cell-free DNA (cfDNA)
by: Katherin Patsch, et al.
Published: (2019-05-01)

Enhanced detection of cell-free DNA (cfDNA) enables its use as a reliable biomarker for diagnosis and prognosis of gastric cancer.
by: Jiyoon Bu, et al.
Published: (2020-01-01)

Genetic alteration and mutation profiling of circulating cell-free tumor DNA (cfDNA) for diagnosis and targeted therapy of gastrointestinal stromal tumors
by: Weixin Yan, et al.
Published: (2016-07-01)

Accuracy of analysis of cfDNA for detection of single nucleotide variants and copy number variants in breast cancer
by: Xin Yang, et al.
Published: (2019-05-01)

Sequencing Shorter cfDNA Fragments Decreases the False Negative Rate of Non-invasive Prenatal Testing
by: Ying Xue, et al.
Published: (2020-03-01)

Kinetics of plasma cfDNA predicts clinical response in non-small cell lung cancer patients
by: Xiaorong Zhou, et al.
Published: (2021-04-01)

Circulating Tumor Cells (CTC) and Cell-Free DNA (cfDNA) Workshop 2016: Scientific Opportunities and Logistics for Cancer Clinical Trial Incorporation
by: Lori E. Lowes, et al.
Published: (2016-09-01)

Downstream methylation analysis of cell-free DNA (cfDNA) from lung cancer patients' plasma following a microfluidic-assisted filtration
by: Duarte Manuel Neves Seixas
Published: (2021)

Mutational monitoring of EGFR T790M in cfDNA for clinical outcome prediction in EGFR-mutant lung adenocarcinoma.
by: Kang-Yi Su, et al.
Published: (2018-01-01)

SinoDuplex: An Improved Duplex Sequencing Approach to Detect Low-frequency Variants in Plasma cfDNA Samples
by: Yongzhe Ren, et al.
Published: (2020-02-01)

cfDNA deconvolution via NIPT of a pregnant woman after bone marrow transplant and donor egg IVF
by: Jianjiang Zhu, et al.
Published: (2021-02-01)

Saliva‐derived cfDNA is applicable for EGFR mutation detection but not for quantitation analysis in non‐small cell lung cancer
by: Shanshan Ding, et al.
Published: (2019-10-01)

Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
by: Keita Tanaka, et al.
Published: (2021-06-01)

Magnetic forces and DNA mechanics in multiplexed magnetic tweezers.
by: Iwijn De Vlaminck, et al.
Published: (2012-01-01)

A new era in aneuploidy screening: cfDNA testing in >30,000 multifetal gestations: Experience at one clinical laboratory.
by: Brittany Dyr, et al.
Published: (2019-01-01)

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients
by: Haiyan E. Liu, et al.
Published: (2020-10-01)

Identification of the plasma total cfDNA level before and after chemotherapy as an indicator of the neoadjuvant chemotherapy response in locally advanced breast cancer
by: Ge Ma, et al.
Published: (2020-04-01)

Performance of the Oncomine<sup>TM</sup> Lung cfDNA Assay for Liquid Biopsy by NGS of NSCLC Patients in Routine Laboratory Practice
by: Giuseppa De Luca, et al.
Published: (2020-04-01)

Development of a Method to Implement Whole-Genome Bisulfite Sequencing of cfDNA from Cancer Patients and a Mouse Tumor Model
by: Elaine C. Maggi, et al.
Published: (2018-01-01)