Application of the adenosine triphosphate sensitivity assay in infantile vascular anomalies

• Main Authors: Li Li, Bin Yang, Li Wei, Bin Zhang, Xiao-feng Han, Zi-gang Xu, Lin Ma
• Format: Article
• Language: English
• Published: BMC 2020-02-01
• Series: BMC Pediatrics
• Subjects: Vascular anomalies; Infantile hemangiomas; Capillary malformations; Adenosine triphosphate; Sensitivity assay; In vitro
• Online Access: http://link.springer.com/article/10.1186/s12887-020-1974-9

Abstract Background The term vascular anomalies include various vascular tumors and vascular malformations, among them infantile hemangiomas and capillary malformations are the most well-known associated diseases in early ages. Multiple drugs have been introduced for intervention, but susceptibility test in vitro were scarcely reported. Objective To evaluate the inhibition effect of different drugs by adenosine triphosphate sensitivity assay in vitro before the treatment of infantile hemangiomas and capillary malformations. Methods Specimens were selected from 5 cases of infantile hemangiomas and 11 cases of capillary malformations. Propranolol, rapamycin, sildenafil and itraconazole were tested for their growth inhibition effect by using the adenosine triphosphate sensitivity assay. Results Propranolol demonstrated inhibitory effects on infantile hemangiomas cells. Rapamycin and itraconazole both showed inhibitory effects on infantile hemangiomas cells and capillary malformations cells. Sildenafil has no growth inhibitory effect on infantile hemangiomas cells or capillary malformations cells. Conclusion Adenosine triphosphate sensitivity assay is a sensitive and useful testing method before the management of vascular anomalies, and individualized medication suggestions for the choice of therapeutic drugs were offered based on the testing result and together with a comprehensive evaluation of each infant.