Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses.
Previously, we showed that mesenchymal stem cells (MSC) can be mobilized into peripheral blood using electroacupuncture (EA) at acupoints, LI-4, LI-11, GV-14, and GV-20. The purpose of this study was to determine whether EA-mobilized MSC could be harvested and expanded in vitro to be used as an auto...
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doaj-03c911fffca74ccd994997d97617e4f12021-03-03T20:49:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021264210.1371/journal.pone.0212642Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses.Ana Leda F LonghiniTatiana E SalazarCristiano VieiraThao TrinhYaqian DuanLouise M PaySergio Li CalziMegan LoshNancy A JohnstonHuisheng XieMinsu KimRobert J HuntMervin C YoderDomenico SantoroTaralyn M McCarrelMaria B GrantPreviously, we showed that mesenchymal stem cells (MSC) can be mobilized into peripheral blood using electroacupuncture (EA) at acupoints, LI-4, LI-11, GV-14, and GV-20. The purpose of this study was to determine whether EA-mobilized MSC could be harvested and expanded in vitro to be used as an autologous cell therapy in horses. Peripheral blood mononuclear cells (PBMC) isolated from young and aged lame horses (n = 29) showed a marked enrichment for MSCs. MSC were expanded in vitro (n = 25) and administered intravenously at a dose of 50 x 106 (n = 24). Treatment resulted in significant improvement in lameness as assessed by the American Association of Equine Practitioners (AAEP) lameness scale (n = 23). MSCs exhibited immunomodulatory function by inhibition of lymphocyte proliferation and induction of IL-10. Intradermal testing showed no immediate or delayed immune reactions to MSC (1 x 106 to 1 x 104). In this study, we demonstrated an efficient, safe and reproducible method to mobilize and expand, in vitro, MSCs in sufficiently high concentrations for therapeutic administration. We confirm the immunomodulatory function of these cells in vitro. This non-pharmacological and non-surgical strategy for stem cell harvest has a broad range of biomedical applications and represents an improved clinically translatable and economical cell source for humans.https://doi.org/10.1371/journal.pone.0212642 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Leda F Longhini Tatiana E Salazar Cristiano Vieira Thao Trinh Yaqian Duan Louise M Pay Sergio Li Calzi Megan Losh Nancy A Johnston Huisheng Xie Minsu Kim Robert J Hunt Mervin C Yoder Domenico Santoro Taralyn M McCarrel Maria B Grant |
spellingShingle |
Ana Leda F Longhini Tatiana E Salazar Cristiano Vieira Thao Trinh Yaqian Duan Louise M Pay Sergio Li Calzi Megan Losh Nancy A Johnston Huisheng Xie Minsu Kim Robert J Hunt Mervin C Yoder Domenico Santoro Taralyn M McCarrel Maria B Grant Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. PLoS ONE |
author_facet |
Ana Leda F Longhini Tatiana E Salazar Cristiano Vieira Thao Trinh Yaqian Duan Louise M Pay Sergio Li Calzi Megan Losh Nancy A Johnston Huisheng Xie Minsu Kim Robert J Hunt Mervin C Yoder Domenico Santoro Taralyn M McCarrel Maria B Grant |
author_sort |
Ana Leda F Longhini |
title |
Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. |
title_short |
Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. |
title_full |
Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. |
title_fullStr |
Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. |
title_full_unstemmed |
Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. |
title_sort |
peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Previously, we showed that mesenchymal stem cells (MSC) can be mobilized into peripheral blood using electroacupuncture (EA) at acupoints, LI-4, LI-11, GV-14, and GV-20. The purpose of this study was to determine whether EA-mobilized MSC could be harvested and expanded in vitro to be used as an autologous cell therapy in horses. Peripheral blood mononuclear cells (PBMC) isolated from young and aged lame horses (n = 29) showed a marked enrichment for MSCs. MSC were expanded in vitro (n = 25) and administered intravenously at a dose of 50 x 106 (n = 24). Treatment resulted in significant improvement in lameness as assessed by the American Association of Equine Practitioners (AAEP) lameness scale (n = 23). MSCs exhibited immunomodulatory function by inhibition of lymphocyte proliferation and induction of IL-10. Intradermal testing showed no immediate or delayed immune reactions to MSC (1 x 106 to 1 x 104). In this study, we demonstrated an efficient, safe and reproducible method to mobilize and expand, in vitro, MSCs in sufficiently high concentrations for therapeutic administration. We confirm the immunomodulatory function of these cells in vitro. This non-pharmacological and non-surgical strategy for stem cell harvest has a broad range of biomedical applications and represents an improved clinically translatable and economical cell source for humans. |
url |
https://doi.org/10.1371/journal.pone.0212642 |
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