Isolasi dan analisis gen yang responsif terhadap cekaman kekeringan pada tebu

• Main Authors: Wiwit Budi Widyasari, Bambang Sugiharto, Cahya Ismayadi, Kabul Wahjudi, Untung Murdiyatmo
• Format: Article
• Language: English
• Published: Indonesian Biological Society 2004-12-01
• Series: Journal of Biological Researches
• Subjects: Saccharum officinarum; Drought inducible protein; dip22-cDNA; RT-PCR
• Online Access: http://berkalahayati.org/journal/bb91f73e

This study was aimed to isolate and identify a gene that was responsive to drought stress in sugar cane variety M442-51, a varietythat is widely used in pastures or dry land. Under drought stress, M442-51 showed an enhance expression of several proteins. One ofthe protein, namely dip22, (drought inducible protein, with a molecular weight of 22KDa) was isolated and analyzed. Amino acidsequence of this protein showed some homology with several stressed related proteins (OsAsr 1 from Oryza sativa leaves, ZM Bss1 ofZea mays leaves, LeAsr 1, LeAsr 2 and LeAsr 3 of Lycopersicum lycopersicon leaves). The 343 bp of dip22-cDNA fragment wasobtained using RT PCR from mRNA of sugar cane leaves that has been exposed to drought stress. The results indicated that the genedip22 plays an important role as a regulatory protein that are usually nested within cytoplasmic matrix or in the nucleus.