Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α
Autosomal dominant polycystic kidney disease (ADPKD) is associated with a number of cellular defects such as hyperproliferation, apoptosis, and dedifferentiation. Mutations in polycystin-1 (PC1) account for ∼85% of ADPKD. Here, we showed that wild-type (WT) or mutant PC1 composed of the last five tr...
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Online Access: | http://dx.doi.org/10.1155/2019/2582401 |
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doaj-0411f25118254131bfd523ef93ac28ad2020-11-24T20:43:07ZengHindawi LimitedBioMed Research International2314-61332314-61412019-01-01201910.1155/2019/25824012582401Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56αYan Tang0JungWoo Yang1Wang Zheng2Jingfeng Tang3Xing-Zhen Chen4Jianzheng Yang5Zuocheng Wang6Department of Oncology and Haematology, The Second Hospital, Jilin University, Changchun 130041, ChinaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton T6G 2H7, CanadaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton T6G 2H7, CanadaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430086, ChinaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton T6G 2H7, CanadaDepartment of Radiotherapy, The Second Hospital, Jilin University, Changchun 130041, ChinaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton T6G 2H7, CanadaAutosomal dominant polycystic kidney disease (ADPKD) is associated with a number of cellular defects such as hyperproliferation, apoptosis, and dedifferentiation. Mutations in polycystin-1 (PC1) account for ∼85% of ADPKD. Here, we showed that wild-type (WT) or mutant PC1 composed of the last five transmembrane (TM) domains and the C-terminus (termed PC1-5TMC) inhibits cell proliferation and protein translation, as well as the downstream effectors of mTOR, consistent with previous reports. Knockdown of B56α, a subunit of the protein phosphatase 2A (PP2A) complex, or application of PP2A inhibitor okadaic acid or calyculin A, abolished the inhibitory effect of PC1 and PC1-5TMC on proliferation, indicating that PP2A/B56α mediates the regulation of cell proliferation by PC1. In addition to the phosphorylated S6 and 4EBP1, B56α was also downregulated by PC1 and PC1-5TMC. Furthermore, the downregulation of B56α, which may be mediated by mTOR but not AKT, can account for the dependence of PC1-inhibited proliferation on PP2A.http://dx.doi.org/10.1155/2019/2582401 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Tang JungWoo Yang Wang Zheng Jingfeng Tang Xing-Zhen Chen Jianzheng Yang Zuocheng Wang |
spellingShingle |
Yan Tang JungWoo Yang Wang Zheng Jingfeng Tang Xing-Zhen Chen Jianzheng Yang Zuocheng Wang Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α BioMed Research International |
author_facet |
Yan Tang JungWoo Yang Wang Zheng Jingfeng Tang Xing-Zhen Chen Jianzheng Yang Zuocheng Wang |
author_sort |
Yan Tang |
title |
Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α |
title_short |
Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α |
title_full |
Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α |
title_fullStr |
Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α |
title_full_unstemmed |
Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α |
title_sort |
polycystin-1 inhibits cell proliferation through phosphatase pp2a/b56α |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2019-01-01 |
description |
Autosomal dominant polycystic kidney disease (ADPKD) is associated with a number of cellular defects such as hyperproliferation, apoptosis, and dedifferentiation. Mutations in polycystin-1 (PC1) account for ∼85% of ADPKD. Here, we showed that wild-type (WT) or mutant PC1 composed of the last five transmembrane (TM) domains and the C-terminus (termed PC1-5TMC) inhibits cell proliferation and protein translation, as well as the downstream effectors of mTOR, consistent with previous reports. Knockdown of B56α, a subunit of the protein phosphatase 2A (PP2A) complex, or application of PP2A inhibitor okadaic acid or calyculin A, abolished the inhibitory effect of PC1 and PC1-5TMC on proliferation, indicating that PP2A/B56α mediates the regulation of cell proliferation by PC1. In addition to the phosphorylated S6 and 4EBP1, B56α was also downregulated by PC1 and PC1-5TMC. Furthermore, the downregulation of B56α, which may be mediated by mTOR but not AKT, can account for the dependence of PC1-inhibited proliferation on PP2A. |
url |
http://dx.doi.org/10.1155/2019/2582401 |
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