Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products
The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins...
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doaj-0426d5c528fd45adb6f646e3f146e24e2020-11-25T02:08:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-12-011010.3389/fmicb.2019.02824493299Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat ProductsTereza Gelbíčová0Alžběta Baráková1Alžběta Baráková2Martina Florianová3Ivana Jamborová4Markéta Zelendová5Markéta Zelendová6Lucie Pospíšilová7Ivana Koláčková8Renáta Karpíšková9Department of Bacteriology, Veterinary Research Institute, Brno, CzechiaDepartment of Bacteriology, Veterinary Research Institute, Brno, CzechiaFaculty of Science, Masaryk University, Brno, CzechiaDepartment of Bacteriology, Veterinary Research Institute, Brno, CzechiaFaculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaFaculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaCentral European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaDepartment of Bacteriology, Veterinary Research Institute, Brno, CzechiaDepartment of Bacteriology, Veterinary Research Institute, Brno, CzechiaDepartment of Bacteriology, Veterinary Research Institute, Brno, CzechiaThe global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain.https://www.frontiersin.org/article/10.3389/fmicb.2019.02824/fullcolistinmcr generesistanceEnterobacteriaceaemeatretail |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tereza Gelbíčová Alžběta Baráková Alžběta Baráková Martina Florianová Ivana Jamborová Markéta Zelendová Markéta Zelendová Lucie Pospíšilová Ivana Koláčková Renáta Karpíšková |
spellingShingle |
Tereza Gelbíčová Alžběta Baráková Alžběta Baráková Martina Florianová Ivana Jamborová Markéta Zelendová Markéta Zelendová Lucie Pospíšilová Ivana Koláčková Renáta Karpíšková Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products Frontiers in Microbiology colistin mcr gene resistance Enterobacteriaceae meat retail |
author_facet |
Tereza Gelbíčová Alžběta Baráková Alžběta Baráková Martina Florianová Ivana Jamborová Markéta Zelendová Markéta Zelendová Lucie Pospíšilová Ivana Koláčková Renáta Karpíšková |
author_sort |
Tereza Gelbíčová |
title |
Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products |
title_short |
Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products |
title_full |
Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products |
title_fullStr |
Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products |
title_full_unstemmed |
Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products |
title_sort |
dissemination and comparison of genetic determinants of mcr-mediated colistin resistance in enterobacteriaceae via retailed raw meat products |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-12-01 |
description |
The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain. |
topic |
colistin mcr gene resistance Enterobacteriaceae meat retail |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02824/full |
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