Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives
Ischemia reperfusion injury is a complex process consisting of a seemingly chaotic but actually organized and compartmentalized shutdown of cell function, of which oxidative stress is a key component. Studying oxidative stress, which results in an imbalance between reactive oxygen species (ROS) prod...
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doaj-042fc1eadf6142f2986d450137a2f6a32021-02-28T00:00:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01222366236610.3390/ijms22052366Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and PerspectivesPauline Chazelas0Clara Steichen1Frédéric Favreau2Patrick Trouillas3Patrick Hannaert4Raphaël Thuillier5Sébastien Giraud6Thierry Hauet7Jérôme Guillard8Maintenance Myélinique et Neuropathies Périphériques, Université de Limoges, EA 6309, F-87000 Limoges, FranceINSERM U1082, IRTOMIT, F-86000 Poitiers, FranceMaintenance Myélinique et Neuropathies Périphériques, Université de Limoges, EA 6309, F-87000 Limoges, FranceINSERM U1248, IPPRITT, Université de Limoges, F-87000 Limoges, FranceINSERM U1082, IRTOMIT, F-86000 Poitiers, FranceINSERM U1082, IRTOMIT, F-86000 Poitiers, FranceINSERM U1082, IRTOMIT, F-86000 Poitiers, FranceINSERM U1082, IRTOMIT, F-86000 Poitiers, FranceUMR CNRS 7285 IC2MP, Team 5 Chemistry, Université de Poitiers, F-86000 Poitiers, FranceIschemia reperfusion injury is a complex process consisting of a seemingly chaotic but actually organized and compartmentalized shutdown of cell function, of which oxidative stress is a key component. Studying oxidative stress, which results in an imbalance between reactive oxygen species (ROS) production and antioxidant defense activity, is a multi-faceted issue, particularly considering the double function of ROS, assuming roles as physiological intracellular signals and as mediators of cellular component damage. Herein, we propose a comprehensive overview of the tools available to explore oxidative stress, particularly in the study of ischemia reperfusion. Applying chemistry as well as biology, we present the different models currently developed to study oxidative stress, spanning the vitro and the silico, discussing the advantages and the drawbacks of each set-up, including the issues relating to the use of in vitro hypoxia as a surrogate for ischemia. Having identified the limitations of historical models, we shall study new paradigms, including the use of stem cell-derived organoids, as a bridge between the in vitro and the in vivo comprising 3D intercellular interactions in vivo and versatile pathway investigations in vitro. We shall conclude this review by distancing ourselves from “wet” biology and reviewing the in silico, computer-based, mathematical modeling, and numerical simulation options: (a) molecular modeling with quantum chemistry and molecular dynamic algorithms, which facilitates the study of molecule-to-molecule interactions, and the integration of a compound in a dynamic environment (the plasma membrane...); (b) integrative systemic models, which can include many facets of complex mechanisms such as oxidative stress or ischemia reperfusion and help to formulate integrated predictions and to enhance understanding of dynamic interaction between pathways.https://www.mdpi.com/1422-0067/22/5/2366oxidative stressROSantioxidant factorsischemia-reperfusion injuryanimal modelsorganoids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pauline Chazelas Clara Steichen Frédéric Favreau Patrick Trouillas Patrick Hannaert Raphaël Thuillier Sébastien Giraud Thierry Hauet Jérôme Guillard |
spellingShingle |
Pauline Chazelas Clara Steichen Frédéric Favreau Patrick Trouillas Patrick Hannaert Raphaël Thuillier Sébastien Giraud Thierry Hauet Jérôme Guillard Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives International Journal of Molecular Sciences oxidative stress ROS antioxidant factors ischemia-reperfusion injury animal models organoids |
author_facet |
Pauline Chazelas Clara Steichen Frédéric Favreau Patrick Trouillas Patrick Hannaert Raphaël Thuillier Sébastien Giraud Thierry Hauet Jérôme Guillard |
author_sort |
Pauline Chazelas |
title |
Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives |
title_short |
Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives |
title_full |
Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives |
title_fullStr |
Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives |
title_full_unstemmed |
Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives |
title_sort |
oxidative stress evaluation in ischemia reperfusion models: characteristics, limits and perspectives |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-02-01 |
description |
Ischemia reperfusion injury is a complex process consisting of a seemingly chaotic but actually organized and compartmentalized shutdown of cell function, of which oxidative stress is a key component. Studying oxidative stress, which results in an imbalance between reactive oxygen species (ROS) production and antioxidant defense activity, is a multi-faceted issue, particularly considering the double function of ROS, assuming roles as physiological intracellular signals and as mediators of cellular component damage. Herein, we propose a comprehensive overview of the tools available to explore oxidative stress, particularly in the study of ischemia reperfusion. Applying chemistry as well as biology, we present the different models currently developed to study oxidative stress, spanning the vitro and the silico, discussing the advantages and the drawbacks of each set-up, including the issues relating to the use of in vitro hypoxia as a surrogate for ischemia. Having identified the limitations of historical models, we shall study new paradigms, including the use of stem cell-derived organoids, as a bridge between the in vitro and the in vivo comprising 3D intercellular interactions in vivo and versatile pathway investigations in vitro. We shall conclude this review by distancing ourselves from “wet” biology and reviewing the in silico, computer-based, mathematical modeling, and numerical simulation options: (a) molecular modeling with quantum chemistry and molecular dynamic algorithms, which facilitates the study of molecule-to-molecule interactions, and the integration of a compound in a dynamic environment (the plasma membrane...); (b) integrative systemic models, which can include many facets of complex mechanisms such as oxidative stress or ischemia reperfusion and help to formulate integrated predictions and to enhance understanding of dynamic interaction between pathways. |
topic |
oxidative stress ROS antioxidant factors ischemia-reperfusion injury animal models organoids |
url |
https://www.mdpi.com/1422-0067/22/5/2366 |
work_keys_str_mv |
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