High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue
The endocannabinoid system (ECS) controls energy balance by regulating both energy intake and energy expenditure. Endocannabinoid levels are elevated in obesity suggesting a potential causal relationship. This study aimed to elucidate the rate of dysregulation of the ECS, and the metabolic organs in...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-01-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01913/full |
| id |
doaj-04969df25b384eee81b3288c63b30ff2 |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Eline N. Kuipers Eline N. Kuipers Vasudev Kantae Boukje C. Eveleens Maarse Boukje C. Eveleens Maarse Susan M. van den Berg Robin van Eenige Robin van Eenige Kimberly J. Nahon Kimberly J. Nahon Anne Reifel-Miller Tamer Coskun Menno P. J. de Winther Esther Lutgens Esther Lutgens Sander Kooijman Sander Kooijman Sander Kooijman Amy C. Harms Thomas Hankemeier Mario van der Stelt Patrick C. N. Rensen Patrick C. N. Rensen Mariëtte R. Boon Mariëtte R. Boon |
| spellingShingle |
Eline N. Kuipers Eline N. Kuipers Vasudev Kantae Boukje C. Eveleens Maarse Boukje C. Eveleens Maarse Susan M. van den Berg Robin van Eenige Robin van Eenige Kimberly J. Nahon Kimberly J. Nahon Anne Reifel-Miller Tamer Coskun Menno P. J. de Winther Esther Lutgens Esther Lutgens Sander Kooijman Sander Kooijman Sander Kooijman Amy C. Harms Thomas Hankemeier Mario van der Stelt Patrick C. N. Rensen Patrick C. N. Rensen Mariëtte R. Boon Mariëtte R. Boon High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue Frontiers in Physiology brown adipose tissue white adipose tissue diet-induced obesity endocannabinoids NAPE-PLD |
| author_facet |
Eline N. Kuipers Eline N. Kuipers Vasudev Kantae Boukje C. Eveleens Maarse Boukje C. Eveleens Maarse Susan M. van den Berg Robin van Eenige Robin van Eenige Kimberly J. Nahon Kimberly J. Nahon Anne Reifel-Miller Tamer Coskun Menno P. J. de Winther Esther Lutgens Esther Lutgens Sander Kooijman Sander Kooijman Sander Kooijman Amy C. Harms Thomas Hankemeier Mario van der Stelt Patrick C. N. Rensen Patrick C. N. Rensen Mariëtte R. Boon Mariëtte R. Boon |
| author_sort |
Eline N. Kuipers |
| title |
High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue |
| title_short |
High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue |
| title_full |
High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue |
| title_fullStr |
High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue |
| title_full_unstemmed |
High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue |
| title_sort |
high fat diet increases circulating endocannabinoids accompanied by increased synthesis enzymes in adipose tissue |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Physiology |
| issn |
1664-042X |
| publishDate |
2019-01-01 |
| description |
The endocannabinoid system (ECS) controls energy balance by regulating both energy intake and energy expenditure. Endocannabinoid levels are elevated in obesity suggesting a potential causal relationship. This study aimed to elucidate the rate of dysregulation of the ECS, and the metabolic organs involved, in diet-induced obesity. Eight groups of age-matched male C57Bl/6J mice were randomized to receive a chow diet (control) or receive a high fat diet (HFD, 45% of calories derived from fat) ranging from 1 day up to 18 weeks before euthanasia. Plasma levels of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (N-arachidonoylethanolamine, AEA), and related N-acylethanolamines, were quantified by UPLC-MS/MS and gene expression of components of the ECS was determined in liver, muscle, white adipose tissue (WAT) and brown adipose tissue (BAT) during the course of diet-induced obesity development. HFD feeding gradually increased 2-AG (+132% within 4 weeks, P < 0.05), accompanied by upregulated expression of its synthesizing enzymes Daglα and β in WAT and BAT. HFD also rapidly increased AEA (+81% within 1 week, P < 0.01), accompanied by increased expression of its synthesizing enzyme Nape-pld, specifically in BAT. Interestingly, Nape-pld expression in BAT correlated with plasma AEA levels (R2 = 0.171, β = 0.276, P < 0.001). We conclude that a HFD rapidly activates adipose tissue depots to increase the synthesis pathways of endocannabinoids that may aggravate the development of HFD-induced obesity. |
| topic |
brown adipose tissue white adipose tissue diet-induced obesity endocannabinoids NAPE-PLD |
| url |
https://www.frontiersin.org/article/10.3389/fphys.2018.01913/full |
| work_keys_str_mv |
AT elinenkuipers highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT elinenkuipers highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT vasudevkantae highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT boukjeceveleensmaarse highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT boukjeceveleensmaarse highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT susanmvandenberg highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT robinvaneenige highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT robinvaneenige highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT kimberlyjnahon highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT kimberlyjnahon highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT annereifelmiller highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT tamercoskun highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT mennopjdewinther highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT estherlutgens highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT estherlutgens highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT sanderkooijman highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT sanderkooijman highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT sanderkooijman highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT amycharms highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT thomashankemeier highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT mariovanderstelt highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT patrickcnrensen highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT patrickcnrensen highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT marietterboon highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue AT marietterboon highfatdietincreasescirculatingendocannabinoidsaccompaniedbyincreasedsynthesisenzymesinadiposetissue |
| _version_ |
1725662425454215168 |
| spelling |
doaj-04969df25b384eee81b3288c63b30ff22020-11-24T22:53:41ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-01-01910.3389/fphys.2018.01913417815High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose TissueEline N. Kuipers0Eline N. Kuipers1Vasudev Kantae2Boukje C. Eveleens Maarse3Boukje C. Eveleens Maarse4Susan M. van den Berg5Robin van Eenige6Robin van Eenige7Kimberly J. Nahon8Kimberly J. Nahon9Anne Reifel-Miller10Tamer Coskun11Menno P. J. de Winther12Esther Lutgens13Esther Lutgens14Sander Kooijman15Sander Kooijman16Sander Kooijman17Amy C. Harms18Thomas Hankemeier19Mario van der Stelt20Patrick C. N. Rensen21Patrick C. N. Rensen22Mariëtte R. Boon23Mariëtte R. Boon24Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsDivision of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Medical Biochemistry, Academic Medical Center, Amsterdam, NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Diabetes/Endocrine, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN, United StatesDepartment of Diabetes/Endocrine, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN, United StatesDepartment of Medical Biochemistry, Academic Medical Center, Amsterdam, NetherlandsDepartment of Medical Biochemistry, Academic Medical Center, Amsterdam, NetherlandsInstitute for Cardiovascular Prevention (IPEK), Ludwig Maximilian University of Munich, Munich, GermanyDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsOxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, United KingdomDivision of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, NetherlandsDivision of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research, Leiden University, Leiden, NetherlandsDepartment of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Leiden, NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, NetherlandsEinthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, NetherlandsThe endocannabinoid system (ECS) controls energy balance by regulating both energy intake and energy expenditure. Endocannabinoid levels are elevated in obesity suggesting a potential causal relationship. This study aimed to elucidate the rate of dysregulation of the ECS, and the metabolic organs involved, in diet-induced obesity. Eight groups of age-matched male C57Bl/6J mice were randomized to receive a chow diet (control) or receive a high fat diet (HFD, 45% of calories derived from fat) ranging from 1 day up to 18 weeks before euthanasia. Plasma levels of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (N-arachidonoylethanolamine, AEA), and related N-acylethanolamines, were quantified by UPLC-MS/MS and gene expression of components of the ECS was determined in liver, muscle, white adipose tissue (WAT) and brown adipose tissue (BAT) during the course of diet-induced obesity development. HFD feeding gradually increased 2-AG (+132% within 4 weeks, P < 0.05), accompanied by upregulated expression of its synthesizing enzymes Daglα and β in WAT and BAT. HFD also rapidly increased AEA (+81% within 1 week, P < 0.01), accompanied by increased expression of its synthesizing enzyme Nape-pld, specifically in BAT. Interestingly, Nape-pld expression in BAT correlated with plasma AEA levels (R2 = 0.171, β = 0.276, P < 0.001). We conclude that a HFD rapidly activates adipose tissue depots to increase the synthesis pathways of endocannabinoids that may aggravate the development of HFD-induced obesity.https://www.frontiersin.org/article/10.3389/fphys.2018.01913/fullbrown adipose tissuewhite adipose tissuediet-induced obesityendocannabinoidsNAPE-PLD |