Genomic approach for conservation and the sustainable management of endangered species of the Amazon.
A broad panel of potentially amplifiable microsatellite loci and a multiplex system were developed for the Amazonian symbol fish species Arapaima gigas, which is currently in high danger of extinction due to the disorderly fishing exploitation. Several factors have contributed to the increase of thi...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0240002 |
id |
doaj-0496c839065b45a48a312a955992d6dc |
---|---|
record_format |
Article |
spelling |
doaj-0496c839065b45a48a312a955992d6dc2021-07-11T04:30:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01162e024000210.1371/journal.pone.0240002Genomic approach for conservation and the sustainable management of endangered species of the Amazon.Paola Fazzi-GomesJonas AguiarGleyce Fonseca CabralDiego MarquesHelber PalhetaFabiano MoreiraMarilia RodriguesRenata CavalcanteJorge SouzaCaio SilvaIgor HamoySidney SantosA broad panel of potentially amplifiable microsatellite loci and a multiplex system were developed for the Amazonian symbol fish species Arapaima gigas, which is currently in high danger of extinction due to the disorderly fishing exploitation. Several factors have contributed to the increase of this threat, among which we highlight the lack of genetic information about the structure and taxonomic status of the species, as well as the lack of accurate tools for evaluation of the effectivity of current management programs. Based on Arapaima gigas' whole genome, available at the NCBI database (ID: 12404), a total of 95,098 unique perfect microsatellites were identified, including their proposed primers. From this panel, a multiplex system containing 12 tetranucleotide microsatellite markers was validated. These tools are valuable for research in as many areas as bioinformatics, ecology, genetics, evolution and comparative studies, since they are able to provide more accurate information for fishing management, conservation of wild populations and genetic management of aquaculture.https://doi.org/10.1371/journal.pone.0240002 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paola Fazzi-Gomes Jonas Aguiar Gleyce Fonseca Cabral Diego Marques Helber Palheta Fabiano Moreira Marilia Rodrigues Renata Cavalcante Jorge Souza Caio Silva Igor Hamoy Sidney Santos |
spellingShingle |
Paola Fazzi-Gomes Jonas Aguiar Gleyce Fonseca Cabral Diego Marques Helber Palheta Fabiano Moreira Marilia Rodrigues Renata Cavalcante Jorge Souza Caio Silva Igor Hamoy Sidney Santos Genomic approach for conservation and the sustainable management of endangered species of the Amazon. PLoS ONE |
author_facet |
Paola Fazzi-Gomes Jonas Aguiar Gleyce Fonseca Cabral Diego Marques Helber Palheta Fabiano Moreira Marilia Rodrigues Renata Cavalcante Jorge Souza Caio Silva Igor Hamoy Sidney Santos |
author_sort |
Paola Fazzi-Gomes |
title |
Genomic approach for conservation and the sustainable management of endangered species of the Amazon. |
title_short |
Genomic approach for conservation and the sustainable management of endangered species of the Amazon. |
title_full |
Genomic approach for conservation and the sustainable management of endangered species of the Amazon. |
title_fullStr |
Genomic approach for conservation and the sustainable management of endangered species of the Amazon. |
title_full_unstemmed |
Genomic approach for conservation and the sustainable management of endangered species of the Amazon. |
title_sort |
genomic approach for conservation and the sustainable management of endangered species of the amazon. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
A broad panel of potentially amplifiable microsatellite loci and a multiplex system were developed for the Amazonian symbol fish species Arapaima gigas, which is currently in high danger of extinction due to the disorderly fishing exploitation. Several factors have contributed to the increase of this threat, among which we highlight the lack of genetic information about the structure and taxonomic status of the species, as well as the lack of accurate tools for evaluation of the effectivity of current management programs. Based on Arapaima gigas' whole genome, available at the NCBI database (ID: 12404), a total of 95,098 unique perfect microsatellites were identified, including their proposed primers. From this panel, a multiplex system containing 12 tetranucleotide microsatellite markers was validated. These tools are valuable for research in as many areas as bioinformatics, ecology, genetics, evolution and comparative studies, since they are able to provide more accurate information for fishing management, conservation of wild populations and genetic management of aquaculture. |
url |
https://doi.org/10.1371/journal.pone.0240002 |
work_keys_str_mv |
AT paolafazzigomes genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT jonasaguiar genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT gleycefonsecacabral genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT diegomarques genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT helberpalheta genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT fabianomoreira genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT mariliarodrigues genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT renatacavalcante genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT jorgesouza genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT caiosilva genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT igorhamoy genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon AT sidneysantos genomicapproachforconservationandthesustainablemanagementofendangeredspeciesoftheamazon |
_version_ |
1721309410504998912 |