Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism
Multidrug resistant (MDR) infections have been a world health issue for many decades, and therefore exploration of new antibiotics to overcome this issue is urgently needed. Regarding antimicrobial properties in the coral holobiont, coral-associated bacteria are suggested as potential producer of ne...
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Elsevier
2019-03-01
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Series: | Egyptian Journal of Aquatic Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S168742851830075X |
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DOAJ |
language |
English |
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Article |
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DOAJ |
author |
Mada Triandala Sibero Tiara Ulfa Bachtiarini Agus Trianto Adindalifa Hayu Lupita Defi Puspita Sari Yasuhiro Igarashi Enjuro Harunari Amit Raj Sharma Ocky Karna Radjasa Agus Sabdono |
spellingShingle |
Mada Triandala Sibero Tiara Ulfa Bachtiarini Agus Trianto Adindalifa Hayu Lupita Defi Puspita Sari Yasuhiro Igarashi Enjuro Harunari Amit Raj Sharma Ocky Karna Radjasa Agus Sabdono Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism Egyptian Journal of Aquatic Research |
author_facet |
Mada Triandala Sibero Tiara Ulfa Bachtiarini Agus Trianto Adindalifa Hayu Lupita Defi Puspita Sari Yasuhiro Igarashi Enjuro Harunari Amit Raj Sharma Ocky Karna Radjasa Agus Sabdono |
author_sort |
Mada Triandala Sibero |
title |
Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism |
title_short |
Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism |
title_full |
Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism |
title_fullStr |
Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism |
title_full_unstemmed |
Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) Organism |
title_sort |
characterization of a yellow pigmented coral-associated bacterium exhibiting anti-bacterial activity against multidrug resistant (mdr) organism |
publisher |
Elsevier |
series |
Egyptian Journal of Aquatic Research |
issn |
1687-4285 |
publishDate |
2019-03-01 |
description |
Multidrug resistant (MDR) infections have been a world health issue for many decades, and therefore exploration of new antibiotics to overcome this issue is urgently needed. Regarding antimicrobial properties in the coral holobiont, coral-associated bacteria are suggested as potential producer of new antibacterial agents against MDR infections. The purposes of this study include isolation and identification of pigmented coral-associated bacteria, preparation of pigmented extract and evaluation of its antibacterial property, as well as the characterization of the pigmented extract using thin layer chromatography (TLC). Nine bacteria candidates were isolated from an unidentified stony coral collected from Tanjung Gelam, Karimunjawa National Park, Jepara Region, Central Java Province, Indonesia. Bacterial pigment and other metabolites were extracted using 1-butanol, ethyl acetate and acetone. Silica gel-based TLC was applied to detect β-carotene and characterize the bacterial crude extracts. Antibacterial activity was evaluated against the extended spectrum β-lactamase (ESBL) E. coli, Klebsiella pneumoniae, and MRSA (methicillin-resistant Staphylococcus aureus). A yellow pigmented bacterium was isolated and identified as Vibrio owensii TNKJ.CR.24-7. Nonribosomal peptide-synthetase Peptide-Synthetase NRPS genes fragments were detected. All bacterial extracts from 1-butanol, ethyl acetate and acetone contained the yellow pigment that was identified as β-carotene. Only the crude extract from ethyl acetate inhibited all of MDR bacteria. TLC chromatogram showed that there were 7 bands from 1-butanol extract and 6 bands from ethyl acetate extract. However, crude extract from acetone was not well separated using silica-based TLC. Bands at Rf 0.64 and 0.81 were found both in 1-butanol and ethyl acetate extracts. Keywords: Antibacterial, β-Carotene, MDR, NRPS, Vibrio |
url |
http://www.sciencedirect.com/science/article/pii/S168742851830075X |
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doaj-04a427d73d364dc7bd9b369d4d8323302020-11-25T00:47:42ZengElsevierEgyptian Journal of Aquatic Research1687-42852019-03-014518187Characterization of a yellow pigmented coral-associated bacterium exhibiting anti-Bacterial Activity Against Multidrug Resistant (MDR) OrganismMada Triandala Sibero0Tiara Ulfa Bachtiarini1Agus Trianto2Adindalifa Hayu Lupita3Defi Puspita Sari4Yasuhiro Igarashi5Enjuro Harunari6Amit Raj Sharma7Ocky Karna Radjasa8Agus Sabdono9Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, Indonesia; Laboratory of Tropical Marine Biotechnology, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Tembalang, Semarang, Central Java, Indonesia; Marine Science Techno Park, Diponegoro University, St. Undip, Teluk Awur Campus, Jepara District, Central Java, Indonesia; Corresponding author at: Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, Indonesia.Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, Indonesia; Laboratory of Tropical Marine Biotechnology, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Tembalang, Semarang, Central Java, IndonesiaDepartment of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, Indonesia; Laboratory of Marine Natural Product, Center for Research and Service, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Tembalang, Semarang, Central Java, IndonesiaDepartment of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, IndonesiaDepartment of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, IndonesiaBiotechnology Research Center, Department of Biotechnology, Toyama Prefectural University, Imizu, Toyama, JapanBiotechnology Research Center, Department of Biotechnology, Toyama Prefectural University, Imizu, Toyama, JapanBiotechnology Research Center, Department of Biotechnology, Toyama Prefectural University, Imizu, Toyama, JapanDepartment of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, Indonesia; Laboratory of Tropical Marine Biotechnology, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Tembalang, Semarang, Central Java, IndonesiaDepartment of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Semarang, Central Java, Indonesia; Laboratory of Tropical Marine Biotechnology, Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang Campus, St. Prof. Soedarto, SH., Tembalang, Semarang, Central Java, IndonesiaMultidrug resistant (MDR) infections have been a world health issue for many decades, and therefore exploration of new antibiotics to overcome this issue is urgently needed. Regarding antimicrobial properties in the coral holobiont, coral-associated bacteria are suggested as potential producer of new antibacterial agents against MDR infections. The purposes of this study include isolation and identification of pigmented coral-associated bacteria, preparation of pigmented extract and evaluation of its antibacterial property, as well as the characterization of the pigmented extract using thin layer chromatography (TLC). Nine bacteria candidates were isolated from an unidentified stony coral collected from Tanjung Gelam, Karimunjawa National Park, Jepara Region, Central Java Province, Indonesia. Bacterial pigment and other metabolites were extracted using 1-butanol, ethyl acetate and acetone. Silica gel-based TLC was applied to detect β-carotene and characterize the bacterial crude extracts. Antibacterial activity was evaluated against the extended spectrum β-lactamase (ESBL) E. coli, Klebsiella pneumoniae, and MRSA (methicillin-resistant Staphylococcus aureus). A yellow pigmented bacterium was isolated and identified as Vibrio owensii TNKJ.CR.24-7. Nonribosomal peptide-synthetase Peptide-Synthetase NRPS genes fragments were detected. All bacterial extracts from 1-butanol, ethyl acetate and acetone contained the yellow pigment that was identified as β-carotene. Only the crude extract from ethyl acetate inhibited all of MDR bacteria. TLC chromatogram showed that there were 7 bands from 1-butanol extract and 6 bands from ethyl acetate extract. However, crude extract from acetone was not well separated using silica-based TLC. Bands at Rf 0.64 and 0.81 were found both in 1-butanol and ethyl acetate extracts. Keywords: Antibacterial, β-Carotene, MDR, NRPS, Vibriohttp://www.sciencedirect.com/science/article/pii/S168742851830075X |