DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells
The chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications an...
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doaj-04ae1270d42a43f8a5554e1a955a041e2021-07-23T14:01:52ZengMDPI AGPlants2223-77472021-07-01101408140810.3390/plants10071408DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> CellsAdrianna Wiktoria Bara0Agnieszka Braszewska1Jolanta Kwasniewska2Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, PolandPlant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, PolandPlant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, PolandThe chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications and the instability of the <i>Brachypodium distachyon</i> genome, which are observed as micronuclei, following maleic hydrazide (MH) and nitroso-N-methylurea (MNU) treatments. We compared the level of DNA methylation in the control (untreated) and mutagen-treated <i>B. distachyon</i> nuclei. An immunostaining method using specific antibodies against modified DNA anti-5-methylcytosine was used for the evaluation of DNA methylation in a single nucleus and micronucleus. Interestingly, we showed an alteration of DNA methylation in cells after mutagenic treatments. The results indicate that DNA methylation might be involved in the response of the <i>B. distachyon</i> genome to mutagenic treatments. This demonstrates that analyses of the epigenetic modifications should be integrated into current plant genetic toxicology in order to explain the mechanisms of DNA damage and repair in plants.https://www.mdpi.com/2223-7747/10/7/1408<i>Brachypodium</i>DNA methylationmaleic hydrazidemicronucleiN-nitroso-N-methylurea |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adrianna Wiktoria Bara Agnieszka Braszewska Jolanta Kwasniewska |
spellingShingle |
Adrianna Wiktoria Bara Agnieszka Braszewska Jolanta Kwasniewska DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells Plants <i>Brachypodium</i> DNA methylation maleic hydrazide micronuclei N-nitroso-N-methylurea |
author_facet |
Adrianna Wiktoria Bara Agnieszka Braszewska Jolanta Kwasniewska |
author_sort |
Adrianna Wiktoria Bara |
title |
DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells |
title_short |
DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells |
title_full |
DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells |
title_fullStr |
DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells |
title_full_unstemmed |
DNA Methylation—An Epigenetic Mark in Mutagen-Treated <i>Brachypodium distachyon</i> Cells |
title_sort |
dna methylation—an epigenetic mark in mutagen-treated <i>brachypodium distachyon</i> cells |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2021-07-01 |
description |
The chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications and the instability of the <i>Brachypodium distachyon</i> genome, which are observed as micronuclei, following maleic hydrazide (MH) and nitroso-N-methylurea (MNU) treatments. We compared the level of DNA methylation in the control (untreated) and mutagen-treated <i>B. distachyon</i> nuclei. An immunostaining method using specific antibodies against modified DNA anti-5-methylcytosine was used for the evaluation of DNA methylation in a single nucleus and micronucleus. Interestingly, we showed an alteration of DNA methylation in cells after mutagenic treatments. The results indicate that DNA methylation might be involved in the response of the <i>B. distachyon</i> genome to mutagenic treatments. This demonstrates that analyses of the epigenetic modifications should be integrated into current plant genetic toxicology in order to explain the mechanisms of DNA damage and repair in plants. |
topic |
<i>Brachypodium</i> DNA methylation maleic hydrazide micronuclei N-nitroso-N-methylurea |
url |
https://www.mdpi.com/2223-7747/10/7/1408 |
work_keys_str_mv |
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1721286274788098048 |