Oligodendrocyte plasticity with an intact cell body in vitro.
Demyelination is generally regarded as a consequence of oligodendrocytic cell death. Oligodendrocyte processes that form myelin sheaths may, however, degenerate and regenerate independently of the cell body, in which case cell death does not necessarily occur. We provide here the first evidence of r...
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doaj-04b2c59c936b4fd1b5c5595f30b349bf2020-11-25T02:42:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6612410.1371/journal.pone.0066124Oligodendrocyte plasticity with an intact cell body in vitro.Manabu MakinodanAya Okuda-YamamotoDaisuke IkawaMichihiro ToritsukaTomohiko TakedaSohei KimotoKouko TatsumiHiroaki OkudaYu NakamuraAkio WanakaToshifumi KishimotoDemyelination is generally regarded as a consequence of oligodendrocytic cell death. Oligodendrocyte processes that form myelin sheaths may, however, degenerate and regenerate independently of the cell body, in which case cell death does not necessarily occur. We provide here the first evidence of retraction and regeneration of oligodendrocyte processes with no cell death in vitro, using time-lapse imaging. When processes were severed mechanically in vitro, the cells did not undergo cell death and the processes regenerated in 36 h. In a separate experiment, moderate N-methyl-D-aspartate (NMDA) stimuli caused process retraction without apparent cell death, and the processes regained their elaborate morphology after NMDA was removed from the culture medium. These results strongly suggest that demyelination and remyelination can take place without concomitant cell death, at least in vitro. Process regeneration may therefore become a target for future therapy of demyelinating disorders.http://europepmc.org/articles/PMC3676349?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manabu Makinodan Aya Okuda-Yamamoto Daisuke Ikawa Michihiro Toritsuka Tomohiko Takeda Sohei Kimoto Kouko Tatsumi Hiroaki Okuda Yu Nakamura Akio Wanaka Toshifumi Kishimoto |
spellingShingle |
Manabu Makinodan Aya Okuda-Yamamoto Daisuke Ikawa Michihiro Toritsuka Tomohiko Takeda Sohei Kimoto Kouko Tatsumi Hiroaki Okuda Yu Nakamura Akio Wanaka Toshifumi Kishimoto Oligodendrocyte plasticity with an intact cell body in vitro. PLoS ONE |
author_facet |
Manabu Makinodan Aya Okuda-Yamamoto Daisuke Ikawa Michihiro Toritsuka Tomohiko Takeda Sohei Kimoto Kouko Tatsumi Hiroaki Okuda Yu Nakamura Akio Wanaka Toshifumi Kishimoto |
author_sort |
Manabu Makinodan |
title |
Oligodendrocyte plasticity with an intact cell body in vitro. |
title_short |
Oligodendrocyte plasticity with an intact cell body in vitro. |
title_full |
Oligodendrocyte plasticity with an intact cell body in vitro. |
title_fullStr |
Oligodendrocyte plasticity with an intact cell body in vitro. |
title_full_unstemmed |
Oligodendrocyte plasticity with an intact cell body in vitro. |
title_sort |
oligodendrocyte plasticity with an intact cell body in vitro. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Demyelination is generally regarded as a consequence of oligodendrocytic cell death. Oligodendrocyte processes that form myelin sheaths may, however, degenerate and regenerate independently of the cell body, in which case cell death does not necessarily occur. We provide here the first evidence of retraction and regeneration of oligodendrocyte processes with no cell death in vitro, using time-lapse imaging. When processes were severed mechanically in vitro, the cells did not undergo cell death and the processes regenerated in 36 h. In a separate experiment, moderate N-methyl-D-aspartate (NMDA) stimuli caused process retraction without apparent cell death, and the processes regained their elaborate morphology after NMDA was removed from the culture medium. These results strongly suggest that demyelination and remyelination can take place without concomitant cell death, at least in vitro. Process regeneration may therefore become a target for future therapy of demyelinating disorders. |
url |
http://europepmc.org/articles/PMC3676349?pdf=render |
work_keys_str_mv |
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