Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities.
Inhibiting ErbB2 signaling with monoclonal antibodies (mAbs) or small molecules is an established therapeutic strategy in oncology. We have developed anti-ErbB2 Dual Variable Domain Immunoglobulin (DVD-Ig) proteins that capture the function of a combination of two anti-ErbB2 antibodies. In addition,...
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doaj-04b66a2bcde548e28545c752fc2bb0672021-03-03T20:14:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9729210.1371/journal.pone.0097292Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities.Jinming GuJinsong YangQing ChangXiaoqing LuJieyi WangMingjiu ChenTariq GhayurJijie GuInhibiting ErbB2 signaling with monoclonal antibodies (mAbs) or small molecules is an established therapeutic strategy in oncology. We have developed anti-ErbB2 Dual Variable Domain Immunoglobulin (DVD-Ig) proteins that capture the function of a combination of two anti-ErbB2 antibodies. In addition, some of the anti-ErbB2 DVD-Ig proteins gain the new functions of enhancing ErbB2 signaling and cell proliferation in N87 cells. We further found that two DVD-Ig proteins, DVD687 and DVD688, have two distinct mechanisms of actions in Calu-3 and N87 cells. DVD687 enhances cell cycle progression while DVD688 induces apoptosis in N87 cells. Using a half DVD687, we found that avidity may play a key role in the agonist activity of DVD687 in N87 cells.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24824849/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jinming Gu Jinsong Yang Qing Chang Xiaoqing Lu Jieyi Wang Mingjiu Chen Tariq Ghayur Jijie Gu |
spellingShingle |
Jinming Gu Jinsong Yang Qing Chang Xiaoqing Lu Jieyi Wang Mingjiu Chen Tariq Ghayur Jijie Gu Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. PLoS ONE |
author_facet |
Jinming Gu Jinsong Yang Qing Chang Xiaoqing Lu Jieyi Wang Mingjiu Chen Tariq Ghayur Jijie Gu |
author_sort |
Jinming Gu |
title |
Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. |
title_short |
Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. |
title_full |
Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. |
title_fullStr |
Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. |
title_full_unstemmed |
Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. |
title_sort |
identification of anti-erbb2 dual variable domain immunoglobulin (dvd-ig™) proteins with unique activities. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Inhibiting ErbB2 signaling with monoclonal antibodies (mAbs) or small molecules is an established therapeutic strategy in oncology. We have developed anti-ErbB2 Dual Variable Domain Immunoglobulin (DVD-Ig) proteins that capture the function of a combination of two anti-ErbB2 antibodies. In addition, some of the anti-ErbB2 DVD-Ig proteins gain the new functions of enhancing ErbB2 signaling and cell proliferation in N87 cells. We further found that two DVD-Ig proteins, DVD687 and DVD688, have two distinct mechanisms of actions in Calu-3 and N87 cells. DVD687 enhances cell cycle progression while DVD688 induces apoptosis in N87 cells. Using a half DVD687, we found that avidity may play a key role in the agonist activity of DVD687 in N87 cells. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24824849/pdf/?tool=EBI |
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