Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis
Under normal and stress conditions plant growth require a complex interplay between phytohormones and reactive oxygen species (ROS). However, details of the nature of this crosstalk remain elusive. Here, we demonstrate that PINOID (PID), a serine threonine kinase of the AGC kinase family, perturbs a...
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doaj-04c47d9d8066473397f8d51f636d83be2020-11-25T00:03:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-08-01810.3389/fpls.2017.01308272549Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in ArabidopsisKumud Saini0Hamada AbdElgawad1Hamada AbdElgawad2Marios N. Markakis3Marios N. Markakis4Sébastjen Schoenaers5Han Asard6Els Prinsen7Gerrit T. S. Beemster8Kris Vissenberg9Kris Vissenberg10Integrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumDepartment of Botany, Faculty of Science, Beni-Suef UniversityBeni Suef, EgyptIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumFaculty of Health and Medical Sciences, University of CopenhagenCopenhagen, DenmarkIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumIntegrated Molecular Plant Physiology Research, University of AntwerpAntwerp, BelgiumPlant and Biochemistry and Biotechnology Lab, Department of Agriculture, School of Agriculture, Food and Nutrition, Technological Educational Institute of Crete: University of Applied SciencesHeraklion, GreeceUnder normal and stress conditions plant growth require a complex interplay between phytohormones and reactive oxygen species (ROS). However, details of the nature of this crosstalk remain elusive. Here, we demonstrate that PINOID (PID), a serine threonine kinase of the AGC kinase family, perturbs auxin homeostasis, which in turn modulates rosette growth and induces stress responses in Arabidopsis plants. Arabidopsis mutants and transgenic plants with altered PID expression were used to study the effect on auxin levels and stress-related responses. In the leaves of plants with ectopic PID expression an accumulation of auxin, oxidative burst and disruption of hormonal balance was apparent. Furthermore, PID overexpression led to the accumulation of antioxidant metabolites, while pid knockout mutants showed only moderate changes in stress-related metabolites. These physiological changes in the plants overexpressing PID modulated their response toward external drought and osmotic stress treatments when compared to the wild type. Based on the morphological, transcriptome, and metabolite results, we propose that perturbations in the auxin hormone levels caused by PID overexpression, along with other hormones and ROS downstream, cause antioxidant accumulation and modify growth and stress responses in Arabidopsis. Our data provide further proof for a strong correlation between auxin and stress biology.http://journal.frontiersin.org/article/10.3389/fpls.2017.01308/fullauxinPINOID (PID)reactive oxygen species (ROS)flavonoidsdrought stressosmotic stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kumud Saini Hamada AbdElgawad Hamada AbdElgawad Marios N. Markakis Marios N. Markakis Sébastjen Schoenaers Han Asard Els Prinsen Gerrit T. S. Beemster Kris Vissenberg Kris Vissenberg |
spellingShingle |
Kumud Saini Hamada AbdElgawad Hamada AbdElgawad Marios N. Markakis Marios N. Markakis Sébastjen Schoenaers Han Asard Els Prinsen Gerrit T. S. Beemster Kris Vissenberg Kris Vissenberg Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis Frontiers in Plant Science auxin PINOID (PID) reactive oxygen species (ROS) flavonoids drought stress osmotic stress |
author_facet |
Kumud Saini Hamada AbdElgawad Hamada AbdElgawad Marios N. Markakis Marios N. Markakis Sébastjen Schoenaers Han Asard Els Prinsen Gerrit T. S. Beemster Kris Vissenberg Kris Vissenberg |
author_sort |
Kumud Saini |
title |
Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis |
title_short |
Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis |
title_full |
Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis |
title_fullStr |
Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis |
title_full_unstemmed |
Perturbation of Auxin Homeostasis and Signaling by PINOID Overexpression Induces Stress Responses in Arabidopsis |
title_sort |
perturbation of auxin homeostasis and signaling by pinoid overexpression induces stress responses in arabidopsis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-08-01 |
description |
Under normal and stress conditions plant growth require a complex interplay between phytohormones and reactive oxygen species (ROS). However, details of the nature of this crosstalk remain elusive. Here, we demonstrate that PINOID (PID), a serine threonine kinase of the AGC kinase family, perturbs auxin homeostasis, which in turn modulates rosette growth and induces stress responses in Arabidopsis plants. Arabidopsis mutants and transgenic plants with altered PID expression were used to study the effect on auxin levels and stress-related responses. In the leaves of plants with ectopic PID expression an accumulation of auxin, oxidative burst and disruption of hormonal balance was apparent. Furthermore, PID overexpression led to the accumulation of antioxidant metabolites, while pid knockout mutants showed only moderate changes in stress-related metabolites. These physiological changes in the plants overexpressing PID modulated their response toward external drought and osmotic stress treatments when compared to the wild type. Based on the morphological, transcriptome, and metabolite results, we propose that perturbations in the auxin hormone levels caused by PID overexpression, along with other hormones and ROS downstream, cause antioxidant accumulation and modify growth and stress responses in Arabidopsis. Our data provide further proof for a strong correlation between auxin and stress biology. |
topic |
auxin PINOID (PID) reactive oxygen species (ROS) flavonoids drought stress osmotic stress |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01308/full |
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