Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study
It is well known that G6PD-deficient individuals are highly susceptible to oxidative stress. However, the differences in the degree of metabolic alterations among patients during an oxidative crisis have not been extensively studied. In this study, we applied mathematical modeling to assess the meta...
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doaj-04d864ccbe314c2fb41115061258c47f2021-07-02T05:17:24ZengHindawi LimitedAdvances in Hematology1687-91041687-91122011-01-01201110.1155/2011/398945398945Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation StudyHanae Shimo0Taiko Nishino1Masaru Tomita2Institute for Advanced Biosciences, Keio University, 403-1, Daihoji, Tsuruoka, Yamagata 997-0017, JapanInstitute for Advanced Biosciences, Keio University, 403-1, Daihoji, Tsuruoka, Yamagata 997-0017, JapanInstitute for Advanced Biosciences, Keio University, 403-1, Daihoji, Tsuruoka, Yamagata 997-0017, JapanIt is well known that G6PD-deficient individuals are highly susceptible to oxidative stress. However, the differences in the degree of metabolic alterations among patients during an oxidative crisis have not been extensively studied. In this study, we applied mathematical modeling to assess the metabolic changes in erythrocytes of various G6PD-deficient patients during hydrogen peroxide- (H2O2-) induced perturbation and predict the kinetic properties that elicit redox imbalance after exposure to an oxidative agent. Simulation results showed a discrepancy in the ability to restore regular metabolite levels and redox homeostasis among patients. Two trends were observed in the response of redox status (GSH/GSSG) to oxidative stress, a mild decrease associated with slow recovery and a drastic decline associated with rapid recovery. The former was concluded to apply to patients with severe clinical symptoms. Low 𝑉max and high 𝐾mG6P of G6PD were shown to be kinetic properties that enhance consequent redox imbalance.http://dx.doi.org/10.1155/2011/398945 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanae Shimo Taiko Nishino Masaru Tomita |
spellingShingle |
Hanae Shimo Taiko Nishino Masaru Tomita Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study Advances in Hematology |
author_facet |
Hanae Shimo Taiko Nishino Masaru Tomita |
author_sort |
Hanae Shimo |
title |
Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study |
title_short |
Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study |
title_full |
Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study |
title_fullStr |
Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study |
title_full_unstemmed |
Predicting the Kinetic Properties Associated with Redox Imbalance after Oxidative Crisis in G6PD-Deficient Erythrocytes: A Simulation Study |
title_sort |
predicting the kinetic properties associated with redox imbalance after oxidative crisis in g6pd-deficient erythrocytes: a simulation study |
publisher |
Hindawi Limited |
series |
Advances in Hematology |
issn |
1687-9104 1687-9112 |
publishDate |
2011-01-01 |
description |
It is well known that G6PD-deficient individuals are highly susceptible to oxidative stress. However, the differences in the degree of metabolic alterations among patients during an oxidative crisis have not been extensively studied. In this study, we applied mathematical modeling to assess the metabolic changes in erythrocytes of various G6PD-deficient patients during hydrogen peroxide- (H2O2-) induced perturbation and predict the kinetic properties that elicit redox imbalance after exposure to an oxidative agent. Simulation results showed a discrepancy in the ability to restore regular metabolite levels and redox homeostasis among patients. Two trends were observed in the response of redox status (GSH/GSSG) to oxidative stress, a mild decrease associated with slow recovery and a drastic decline associated with rapid recovery. The former was concluded to apply to patients with severe clinical symptoms. Low 𝑉max and high 𝐾mG6P of G6PD were shown to be kinetic properties that enhance consequent redox imbalance. |
url |
http://dx.doi.org/10.1155/2011/398945 |
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