A Breast Cell Atlas: Organelle analysis of the MDA-MB-231 cell line by density-gradient fractionation using isotopic marking and label-free analysis

A Breast Cell Atlas: Organelle analysis of the MDA-MB-231 cell line by density-gradient fractionation using isotopic marking and label-free analysis

Protein translocation between organelles in the cell is an important process that regulates many cellular functions. However, organelles can rarely be isolated to purity so several methods have been developed to analyse the fractions obtained by density gradient centrifugation. We present an analysi...

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Bibliographic Details
Main Authors: Marianne Sandin, Linn Antberg, Fredrik Levander, Peter James
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:EuPA Open Proteomics
Subjects:
LOPIT (location of organelle proteins by isotopic tagging)
Label-free quantification
Isotopic-labelling
Organelle
Subcellular location
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968515300040

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http://www.sciencedirect.com/science/article/pii/S2212968515300040

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