Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis
Postcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and ce...
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2016-01-01
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doaj-04eb662c50d04e64a335fdbea4d5ba292020-11-24T20:58:46ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/21063422106342Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest AcidosisYan-Ren Lin0Chao-Jui Li1Shih-Han Syu2Cheng-Hao Wen3Waradee Buddhakosai4Han-Ping Wu5Cheng Hsu Chen6Huai-En Lu7Wen-Liang Chen8Department of Emergency Medicine, Changhua Christian Hospital, Changhua, TaiwanDepartment of Emergency Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, TaiwanBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, TaiwanBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, TaiwanDepartment of Biological Science and Technology, National Chiao Tung University, Hsinchu, TaiwanDivision of Pediatric General Medicine, Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Kweishan, Taoyuan, TaiwanDepartment of Emergency Medicine, Changhua Christian Hospital, Changhua, TaiwanBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, TaiwanDepartment of Biological Science and Technology, National Chiao Tung University, Hsinchu, TaiwanPostcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and cell models. Forty Wistar rats with postcardiac arrest acidosis (blood pH < 7.2) were included. They were divided into study (500 mg/kg L-alanyl-L-glutamine, n=20) and control (normal saline, n=20) groups. Each of the rats received resuscitation. The outcomes were compared between the two groups. In addition, cardiomyocytes derived from human induced pluripotent stem cells were exposed to HBSS with different pH levels (7.3 or 6.5) or to culture medium (control). Apoptosis-related markers and beating function were analyzed. We found that the duration of survival was significantly longer in the study group (p<0.05). In addition, in pH 6.5 or pH 7.3 HBSS buffer, the expression levels of cell stress (p53) and apoptosis (caspase-3, Bcl-xL) markers were significantly lower in cardiomyocytes treated with 50 mM L-glutamine than those without L-glutamine (RT-PCR). L-glutamine also increased the beating function of cardiomyocytes, especially at the lower pH level (6.5). More importantly, glutamine decreased cardiomyocyte apoptosis and increased these cells’ beating function at a low pH level.http://dx.doi.org/10.1155/2016/2106342 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan-Ren Lin Chao-Jui Li Shih-Han Syu Cheng-Hao Wen Waradee Buddhakosai Han-Ping Wu Cheng Hsu Chen Huai-En Lu Wen-Liang Chen |
spellingShingle |
Yan-Ren Lin Chao-Jui Li Shih-Han Syu Cheng-Hao Wen Waradee Buddhakosai Han-Ping Wu Cheng Hsu Chen Huai-En Lu Wen-Liang Chen Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis BioMed Research International |
author_facet |
Yan-Ren Lin Chao-Jui Li Shih-Han Syu Cheng-Hao Wen Waradee Buddhakosai Han-Ping Wu Cheng Hsu Chen Huai-En Lu Wen-Liang Chen |
author_sort |
Yan-Ren Lin |
title |
Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis |
title_short |
Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis |
title_full |
Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis |
title_fullStr |
Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis |
title_full_unstemmed |
Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis |
title_sort |
early administration of glutamine protects cardiomyocytes from post-cardiac arrest acidosis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
Postcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and cell models. Forty Wistar rats with postcardiac arrest acidosis (blood pH < 7.2) were included. They were divided into study (500 mg/kg L-alanyl-L-glutamine, n=20) and control (normal saline, n=20) groups. Each of the rats received resuscitation. The outcomes were compared between the two groups. In addition, cardiomyocytes derived from human induced pluripotent stem cells were exposed to HBSS with different pH levels (7.3 or 6.5) or to culture medium (control). Apoptosis-related markers and beating function were analyzed. We found that the duration of survival was significantly longer in the study group (p<0.05). In addition, in pH 6.5 or pH 7.3 HBSS buffer, the expression levels of cell stress (p53) and apoptosis (caspase-3, Bcl-xL) markers were significantly lower in cardiomyocytes treated with 50 mM L-glutamine than those without L-glutamine (RT-PCR). L-glutamine also increased the beating function of cardiomyocytes, especially at the lower pH level (6.5). More importantly, glutamine decreased cardiomyocyte apoptosis and increased these cells’ beating function at a low pH level. |
url |
http://dx.doi.org/10.1155/2016/2106342 |
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