Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease

Objective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital...

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Main Authors: Yu-Fei Xie, Yi-Peng Ding
Format: Article
Language:English
Published: Editorial Board of Journal of Hainan Medical University 2020-12-01
Series:Journal of Hainan Medical University
Subjects:
Online Access:http://www.hnykdxxb.com/PDF/202024/04.pdf
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spelling doaj-04f7e743729245e79d35a79efc8ec5c42021-01-13T08:24:02ZengEditorial Board of Journal of Hainan Medical UniversityJournal of Hainan Medical University1007-12371007-12372020-12-0126241417Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary diseaseYu-Fei Xie0Yi-Peng Ding1Hainan Hospital Affiliated to Hainan Medical University, Haikou 570311, ChinaHainan Hospital Affiliated to Hainan Medical University, Haikou 570311, ChinaObjective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected. Extraction of exosomes and electron microscopy to identify the characteristics of exosomes. RT-PCR method was used to detect the miR-186 expression level in serum exosomes. And statistical analysis of the expression difference between the COPD group and the normal group, alysis of the correlation between miR-186 and FEV1% predicted value, and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD. Results: The relative expression of miR-186 in serum exosomes of COPD group was 3.24 ± 0.14, which was significantly higher than that of normal control group 1.03 ± 0.04 (t = 15.47, P <0.001). The expression level of miR-186 was negatively correlated with the predicted FEV1% of COPD patients (r = -0.5024, p <0.0001). The area under the ROC curve between miR-186 and COPD patients was 0.8409 (95% confidence interval: 0.6875 -0.9943). Conclusion: The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD.http://www.hnykdxxb.com/PDF/202024/04.pdfcopdexosomesmirnabiomarkers
collection DOAJ
language English
format Article
sources DOAJ
author Yu-Fei Xie
Yi-Peng Ding
spellingShingle Yu-Fei Xie
Yi-Peng Ding
Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
Journal of Hainan Medical University
copd
exosomes
mirna
biomarkers
author_facet Yu-Fei Xie
Yi-Peng Ding
author_sort Yu-Fei Xie
title Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
title_short Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
title_full Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
title_fullStr Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
title_full_unstemmed Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
title_sort expression and clinical significance of mir-186 in serum exosomes of patients with chronic obstructive pulmonary disease
publisher Editorial Board of Journal of Hainan Medical University
series Journal of Hainan Medical University
issn 1007-1237
1007-1237
publishDate 2020-12-01
description Objective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected. Extraction of exosomes and electron microscopy to identify the characteristics of exosomes. RT-PCR method was used to detect the miR-186 expression level in serum exosomes. And statistical analysis of the expression difference between the COPD group and the normal group, alysis of the correlation between miR-186 and FEV1% predicted value, and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD. Results: The relative expression of miR-186 in serum exosomes of COPD group was 3.24 ± 0.14, which was significantly higher than that of normal control group 1.03 ± 0.04 (t = 15.47, P <0.001). The expression level of miR-186 was negatively correlated with the predicted FEV1% of COPD patients (r = -0.5024, p <0.0001). The area under the ROC curve between miR-186 and COPD patients was 0.8409 (95% confidence interval: 0.6875 -0.9943). Conclusion: The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD.
topic copd
exosomes
mirna
biomarkers
url http://www.hnykdxxb.com/PDF/202024/04.pdf
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