Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease
Objective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital...
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doaj-04f7e743729245e79d35a79efc8ec5c42021-01-13T08:24:02ZengEditorial Board of Journal of Hainan Medical UniversityJournal of Hainan Medical University1007-12371007-12372020-12-0126241417Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary diseaseYu-Fei Xie0Yi-Peng Ding1Hainan Hospital Affiliated to Hainan Medical University, Haikou 570311, ChinaHainan Hospital Affiliated to Hainan Medical University, Haikou 570311, ChinaObjective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected. Extraction of exosomes and electron microscopy to identify the characteristics of exosomes. RT-PCR method was used to detect the miR-186 expression level in serum exosomes. And statistical analysis of the expression difference between the COPD group and the normal group, alysis of the correlation between miR-186 and FEV1% predicted value, and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD. Results: The relative expression of miR-186 in serum exosomes of COPD group was 3.24 ± 0.14, which was significantly higher than that of normal control group 1.03 ± 0.04 (t = 15.47, P <0.001). The expression level of miR-186 was negatively correlated with the predicted FEV1% of COPD patients (r = -0.5024, p <0.0001). The area under the ROC curve between miR-186 and COPD patients was 0.8409 (95% confidence interval: 0.6875 -0.9943). Conclusion: The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD.http://www.hnykdxxb.com/PDF/202024/04.pdfcopdexosomesmirnabiomarkers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Fei Xie Yi-Peng Ding |
spellingShingle |
Yu-Fei Xie Yi-Peng Ding Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease Journal of Hainan Medical University copd exosomes mirna biomarkers |
author_facet |
Yu-Fei Xie Yi-Peng Ding |
author_sort |
Yu-Fei Xie |
title |
Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease |
title_short |
Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease |
title_full |
Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease |
title_fullStr |
Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease |
title_full_unstemmed |
Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease |
title_sort |
expression and clinical significance of mir-186 in serum exosomes of patients with chronic obstructive pulmonary disease |
publisher |
Editorial Board of Journal of Hainan Medical University |
series |
Journal of Hainan Medical University |
issn |
1007-1237 1007-1237 |
publishDate |
2020-12-01 |
description |
Objective: To study the expression of miR-186 in serum exosomes of patients with chronic
obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive
pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal
persons in our hospital were collected. Extraction of exosomes and electron microscopy to
identify the characteristics of exosomes. RT-PCR method was used to detect the miR-186
expression level in serum exosomes. And statistical analysis of the expression difference
between the COPD group and the normal group, alysis of the correlation between miR-186 and
FEV1% predicted value, and ROC curve analysis of the diagnostic value of serum exosome
miR-186 in COPD. Results: The relative expression of miR-186 in serum exosomes of COPD
group was 3.24 ± 0.14, which was significantly higher than that of normal control group 1.03 ±
0.04 (t = 15.47, P <0.001). The expression level of miR-186 was negatively correlated with the
predicted FEV1% of COPD patients (r = -0.5024, p <0.0001). The area under the ROC curve
between miR-186 and COPD patients was 0.8409 (95% confidence interval: 0.6875 -0.9943).
Conclusion: The high expression of miR-186 in serum exosomes may be a risk factor and may
be used as a diagnostic indicator of COPD. |
topic |
copd exosomes mirna biomarkers |
url |
http://www.hnykdxxb.com/PDF/202024/04.pdf |
work_keys_str_mv |
AT yufeixie expressionandclinicalsignificanceofmir186inserumexosomesofpatientswithchronicobstructivepulmonarydisease AT yipengding expressionandclinicalsignificanceofmir186inserumexosomesofpatientswithchronicobstructivepulmonarydisease |
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