Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle
This research has been conducted at the laboratory of in vitro fertilization in the Department of Animal Science University of Wisconsin, USA. These embryos can be used for improving genetic value of Indonesian cattle. Oocytes were matured in TCM- 199 medium (in 5% CO2 incubator and at 390C) enriche...
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Pusat Penelitian dan Pengembangan Peternakan
2002-03-01
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doaj-0503629db5324a0d8261b5d729b4f3a42020-11-24T22:34:36ZengPusat Penelitian dan Pengembangan PeternakanJurnal Ilmu Ternak dan Veteriner0853-73802252-696X2002-03-01713037Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattleEndang TriwulaninngsihM.R ToelihereJ.J RutledgeT.L YusufB PurwantaraK DiwyantoThis research has been conducted at the laboratory of in vitro fertilization in the Department of Animal Science University of Wisconsin, USA. These embryos can be used for improving genetic value of Indonesian cattle. Oocytes were matured in TCM- 199 medium (in 5% CO2 incubator and at 390C) enriched with follicle stimulating hormone (FSH) 10 μl/ml, oestradiol 17 β 1μl/ml and 10% Fetal Calf Serum (FCS). The oocytes were fertilized in vitro with motile sperm and incubation between sperm and oocytes in fertilization medium Tyroide Albumin Lactate Pyruvate (TALP) for 20 hours. All zygotes were cultured in CR1aa (n=1549) medium versus modification of protein-free pottasium simplex optimized medium (KSOM) (n=675) up to blastocyst stage and were fed FCS 5 μl/50 μl medium on day 6, as treatment A and B respectively. Data were analyzed by completely randomized design with SAS program. Percentages of cleavage, morula, blastocyst, expanded blastocyst, unfertilized and degenerated ova in this study were 91.4% vs 75.6 %; 75.6% vs 58.9%; 61.5% vs 38.5%; 31.2% vs 5.1%, 8.6% vs 24.4%, 15.7% vs 8% which were significantly different (P<0.01) for treatment CR1aa and KSOM respectively. Based on this study, CR1aa medium is better culture medium than KSOM for efficient in vitro production (IVP) of bovine embryos.http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/272/272Oocytesin vitro fertilizationembryoblastocystculture medium |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Endang Triwulaninngsih M.R Toelihere J.J Rutledge T.L Yusuf B Purwantara K Diwyanto |
spellingShingle |
Endang Triwulaninngsih M.R Toelihere J.J Rutledge T.L Yusuf B Purwantara K Diwyanto Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle Jurnal Ilmu Ternak dan Veteriner Oocytes in vitro fertilization embryo blastocyst culture medium |
author_facet |
Endang Triwulaninngsih M.R Toelihere J.J Rutledge T.L Yusuf B Purwantara K Diwyanto |
author_sort |
Endang Triwulaninngsih |
title |
Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle |
title_short |
Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle |
title_full |
Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle |
title_fullStr |
Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle |
title_full_unstemmed |
Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle |
title_sort |
using cr1aa versus ksom as the culture medium for in vitro embryo production of cattle |
publisher |
Pusat Penelitian dan Pengembangan Peternakan |
series |
Jurnal Ilmu Ternak dan Veteriner |
issn |
0853-7380 2252-696X |
publishDate |
2002-03-01 |
description |
This research has been conducted at the laboratory of in vitro fertilization in the Department of Animal Science University of Wisconsin, USA. These embryos can be used for improving genetic value of Indonesian cattle. Oocytes were matured in TCM- 199 medium (in 5% CO2 incubator and at 390C) enriched with follicle stimulating hormone (FSH) 10 μl/ml, oestradiol 17 β 1μl/ml and 10% Fetal Calf Serum (FCS). The oocytes were fertilized in vitro with motile sperm and incubation between sperm and oocytes in fertilization medium Tyroide Albumin Lactate Pyruvate (TALP) for 20 hours. All zygotes were cultured in CR1aa (n=1549) medium versus modification of protein-free pottasium simplex optimized medium (KSOM) (n=675) up to blastocyst stage and were fed FCS 5 μl/50 μl medium on day 6, as treatment A and B respectively. Data were analyzed by completely randomized design with SAS program. Percentages of cleavage, morula, blastocyst, expanded blastocyst, unfertilized and degenerated ova in this study were 91.4% vs 75.6 %; 75.6% vs 58.9%; 61.5% vs 38.5%; 31.2% vs 5.1%, 8.6% vs 24.4%, 15.7% vs 8% which were significantly different (P<0.01) for treatment CR1aa and KSOM respectively. Based on this study, CR1aa medium is better culture medium than KSOM for efficient in vitro production (IVP) of bovine embryos. |
topic |
Oocytes in vitro fertilization embryo blastocyst culture medium |
url |
http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/272/272 |
work_keys_str_mv |
AT endangtriwulaninngsih usingcr1aaversusksomastheculturemediumforinvitroembryoproductionofcattle AT mrtoelihere usingcr1aaversusksomastheculturemediumforinvitroembryoproductionofcattle AT jjrutledge usingcr1aaversusksomastheculturemediumforinvitroembryoproductionofcattle AT tlyusuf usingcr1aaversusksomastheculturemediumforinvitroembryoproductionofcattle AT bpurwantara usingcr1aaversusksomastheculturemediumforinvitroembryoproductionofcattle AT kdiwyanto usingcr1aaversusksomastheculturemediumforinvitroembryoproductionofcattle |
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