Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae
Juvenile hormone analogs (JHA) are known to interfere with growth and biosynthesis of insects with potential for insecticide action. However, there has been comparatively few data on morphological effects of JHA on insect organs. To determine pyriproxyfen effects on Aedes aegypti larvae, we conducte...
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doaj-050a4d4259464835ac65a888bc15e4032020-11-24T22:21:00ZengPeerJ Inc.PeerJ2167-83592019-09-017e748910.7717/peerj.7489Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvaeMuhammad Fiaz0Luis Carlos Martínez1Angelica Plata-Rueda2Wagner Gonzaga Gonçalves3Debora Linhares Lino de Souza4Jamile Fernanda Silva Cossolin5Paulo Eduardo Gomes Rodrigues Carvalho6Gustavo Ferreira Martins7José Eduardo Serrão8Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Entomologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, BrazilJuvenile hormone analogs (JHA) are known to interfere with growth and biosynthesis of insects with potential for insecticide action. However, there has been comparatively few data on morphological effects of JHA on insect organs. To determine pyriproxyfen effects on Aedes aegypti larvae, we conducted toxicity, behavioral bioassays and assessed ultrastructural effects of pyriproxyfen on midgut cells. A. aegypti larvae were exposed in aqueous solution of pyriproxyfen LC50 concentrations and evaluated for 24 h. This study fulfilled the toxic prevalence of pyriproxyfen to A. aegypti larvae (LC50 = 8.2 mg L−1). Behavioral observations confirmed that pyriproxyfen treatment significantly changes swimming behavior of larvae, limiting its displacement and speed. The pyriproxyfen causes remarkable histopathological and cytotoxic alterations in the midgut of larvae. Histopathological study reveals presence of cytoplasmic vacuolization and damage to brush border of the digestive cells. The main salient lesions of cytotoxic effects are occurrence of cell debris released into the midgut lumen, cytoplasm rich in lipid droplets, autophagosomes, disorganized microvilli and deformed mitochondria. Data suggest that pyriproxyfen can be used to help to control and eradicate this insect vector.https://peerj.com/articles/7489.pdfAutophagyAedes aegyptiUltrastructureJuvenile HormoneLocomotory behavior |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Fiaz Luis Carlos Martínez Angelica Plata-Rueda Wagner Gonzaga Gonçalves Debora Linhares Lino de Souza Jamile Fernanda Silva Cossolin Paulo Eduardo Gomes Rodrigues Carvalho Gustavo Ferreira Martins José Eduardo Serrão |
spellingShingle |
Muhammad Fiaz Luis Carlos Martínez Angelica Plata-Rueda Wagner Gonzaga Gonçalves Debora Linhares Lino de Souza Jamile Fernanda Silva Cossolin Paulo Eduardo Gomes Rodrigues Carvalho Gustavo Ferreira Martins José Eduardo Serrão Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae PeerJ Autophagy Aedes aegypti Ultrastructure Juvenile Hormone Locomotory behavior |
author_facet |
Muhammad Fiaz Luis Carlos Martínez Angelica Plata-Rueda Wagner Gonzaga Gonçalves Debora Linhares Lino de Souza Jamile Fernanda Silva Cossolin Paulo Eduardo Gomes Rodrigues Carvalho Gustavo Ferreira Martins José Eduardo Serrão |
author_sort |
Muhammad Fiaz |
title |
Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae |
title_short |
Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae |
title_full |
Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae |
title_fullStr |
Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae |
title_full_unstemmed |
Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae |
title_sort |
pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of aedes aegypti larvae |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2019-09-01 |
description |
Juvenile hormone analogs (JHA) are known to interfere with growth and biosynthesis of insects with potential for insecticide action. However, there has been comparatively few data on morphological effects of JHA on insect organs. To determine pyriproxyfen effects on Aedes aegypti larvae, we conducted toxicity, behavioral bioassays and assessed ultrastructural effects of pyriproxyfen on midgut cells. A. aegypti larvae were exposed in aqueous solution of pyriproxyfen LC50 concentrations and evaluated for 24 h. This study fulfilled the toxic prevalence of pyriproxyfen to A. aegypti larvae (LC50 = 8.2 mg L−1). Behavioral observations confirmed that pyriproxyfen treatment significantly changes swimming behavior of larvae, limiting its displacement and speed. The pyriproxyfen causes remarkable histopathological and cytotoxic alterations in the midgut of larvae. Histopathological study reveals presence of cytoplasmic vacuolization and damage to brush border of the digestive cells. The main salient lesions of cytotoxic effects are occurrence of cell debris released into the midgut lumen, cytoplasm rich in lipid droplets, autophagosomes, disorganized microvilli and deformed mitochondria. Data suggest that pyriproxyfen can be used to help to control and eradicate this insect vector. |
topic |
Autophagy Aedes aegypti Ultrastructure Juvenile Hormone Locomotory behavior |
url |
https://peerj.com/articles/7489.pdf |
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