Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection
ABSTRACT Background and Objectives: Ivermectin is an anti-parasitic medication frequently used in many food-producing animals. This study aimed to investigate the level of ivermectin residue in liver samples collected from slaughterhouses in Qazvin Province, Iran. Methods: Overall...
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Golestan University of Medical Sciences
2017-03-01
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doaj-0516eb23ec99409fb26668258d6c87772021-09-01T13:20:29ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492017-03-011121620Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence DetectionDavoodi Jabber0Reza Norian1Mohammad Jalilvand2 Department Of Pathobiology, Agriculture Faculty, Islamic Azad University Abhar Branch, Abhar, Iran Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Department of Pathobiology, Agriculture Faculty, Islamic Azad University Abhar Branch, Abhar, Iran ABSTRACT Background and Objectives: Ivermectin is an anti-parasitic medication frequently used in many food-producing animals. This study aimed to investigate the level of ivermectin residue in liver samples collected from slaughterhouses in Qazvin Province, Iran. Methods: Overall, 88 bovine liver samples were randomly collected and analyzed for detection of ivermectin residues. The samples were analyzed for ivermectin contamination by enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The samples were extracted using liquid-liquid extraction procedure for the ELISA. Solid phase extraction using a C18 column followed by fluorescence-derivatized with 1-methylimidazole and trifluoroacetic anhydride in acetonitrile were used for the HPLC assay. Recovery values obtained from the HPLC method ranged from 81.3 to 92.5%, with a relative standard deviation of 6.7-12.2%. Results: First, all samples were screened by the ELISA method. Based on the results, 16 samples (18.2%) contained no detectable levels of Ivermectin residue, while Ivermectin was found in 72 samples (81.8%). In addition, 22 of the positive samples (30.55%) contained high Ivermectin level (>50 ppb). Analysis of the samples by the HPLC method showed that eight samples (9.09%) contained ivermectin levels above the maximum residue limit. Conclusion: This study demonstrates the presence of different levels of Ivermectin residue in bovine liver samples collected from the Qazvin Province in Iran. Therefore, regulatory authorities should ensure proper withdrawal period before slaughter of the animals. Keywords: Ivermectin, Cattle, Liver, ELISA, HPLC.http://mlj.goums.ac.ir/article-1-974-en.htmlivermectincattleliverelisahplc. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Davoodi Jabber Reza Norian Mohammad Jalilvand |
spellingShingle |
Davoodi Jabber Reza Norian Mohammad Jalilvand Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection Medical Laboratory Journal ivermectin cattle liver elisa hplc. |
author_facet |
Davoodi Jabber Reza Norian Mohammad Jalilvand |
author_sort |
Davoodi Jabber |
title |
Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection |
title_short |
Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection |
title_full |
Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection |
title_fullStr |
Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection |
title_full_unstemmed |
Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection |
title_sort |
determination of ivermectin residues in bovine liver samples by elisa and hplc with fluorescence detection |
publisher |
Golestan University of Medical Sciences |
series |
Medical Laboratory Journal |
issn |
2538-4449 |
publishDate |
2017-03-01 |
description |
ABSTRACT
Background and Objectives: Ivermectin is an anti-parasitic medication frequently used in many food-producing animals. This study aimed to investigate the level of ivermectin residue in liver samples collected from slaughterhouses in Qazvin Province, Iran.
Methods: Overall, 88 bovine liver samples were randomly collected and analyzed for detection of ivermectin residues. The samples were analyzed for ivermectin contamination by enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The samples were extracted using liquid-liquid extraction procedure for the ELISA. Solid phase extraction using a C18 column followed by fluorescence-derivatized with 1-methylimidazole and trifluoroacetic anhydride in acetonitrile were used for the HPLC assay. Recovery values obtained from the HPLC method ranged from 81.3 to 92.5%, with a relative standard deviation of 6.7-12.2%.
Results: First, all samples were screened by the ELISA method. Based on the results, 16 samples (18.2%) contained no detectable levels of Ivermectin residue, while Ivermectin was found in 72 samples (81.8%). In addition, 22 of the positive samples (30.55%) contained high Ivermectin level (>50 ppb). Analysis of the samples by the HPLC method showed that eight samples (9.09%) contained ivermectin levels above the maximum residue limit.
Conclusion: This study demonstrates the presence of different levels of Ivermectin residue in bovine liver samples collected from the Qazvin Province in Iran. Therefore, regulatory authorities should ensure proper withdrawal period before slaughter of the animals.
Keywords: Ivermectin, Cattle, Liver, ELISA, HPLC. |
topic |
ivermectin cattle liver elisa hplc. |
url |
http://mlj.goums.ac.ir/article-1-974-en.html |
work_keys_str_mv |
AT davoodijabber determinationofivermectinresiduesinbovineliversamplesbyelisaandhplcwithfluorescencedetection AT rezanorian determinationofivermectinresiduesinbovineliversamplesbyelisaandhplcwithfluorescencedetection AT mohammadjalilvand determinationofivermectinresiduesinbovineliversamplesbyelisaandhplcwithfluorescencedetection |
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