Development of an immunochromatography assay for salinomycin and methyl salinomycin in honey
This study developed a sensitive monoclonal antibody, 3A3, to salinomycin (SLM) and methyl salinomycin (MLN). The monoclonal antibody 3A3 is highly specific for SLM with an antibody halfinhibitory concentration (IC 50) of 0.86 ng/mL, a detection limit of 0.28 ng/mL, and a linear detection range of 0...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1649370 |
| Summary: | This study developed a sensitive monoclonal antibody, 3A3, to salinomycin (SLM) and methyl salinomycin (MLN). The monoclonal antibody 3A3 is highly specific for SLM with an antibody halfinhibitory concentration (IC 50) of 0.86 ng/mL, a detection limit of 0.28 ng/mL, and a linear detection range of 0.28–2.26 ng/mL. The cross-reaction values of SLM and MLN were 100% and 74.48%, respectively. The recovery of honey samples ranged from 94.6% to 100.4%, thus indicating that this analytical method can be used for the analysis of real honey samples. In addition, immunochromatographic bands were developed for qualitative, semi-quantitative and speckle detection analysis. The critical values of SLM and MLN in honey were 40 and 40 ng/mL, respectively. The results showed that these analyses can be used for real samples, with the most stringent MRL of 40 ng/mL, for SLM and MLN. |
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| ISSN: | 0954-0105 1465-3443 |