Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model
Psoriasis is an autoimmune inflammatory disease, where dendritic cells (DCs) play an important role in its pathogenesis. In our previous work, we have demonstrated that topical delivery of curcumin-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) could treat Imiquimod (IMQ)-induced p...
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doaj-0527b6d7d885422095bc9d60cecfa4202021-05-04T07:25:52ZengElsevierActa Pharmaceutica Sinica B2211-38352021-04-0111410471055Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice modelZibei Lin0Long Xi1Shaokui Chen2Jinsong Tao3Yan Wang4Xin Chen5Ping Li6Zhenping Wang7Ying Zheng8State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, ChinaBeijing Hospital of Traditional Chinese Medicine, Affiliated with Capital Medical University, Beijing 100050, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, ChinaBeijing Hospital of Traditional Chinese Medicine, Affiliated with Capital Medical University, Beijing 100050, ChinaDepartment of Dermatology, School of Medicine, University of California, La Jolla, San Diego, CA 92093, USAState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China; Corresponding author. Fax: +853 28841358.Psoriasis is an autoimmune inflammatory disease, where dendritic cells (DCs) play an important role in its pathogenesis. In our previous work, we have demonstrated that topical delivery of curcumin-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) could treat Imiquimod (IMQ)-induced psoriasis-like mice. The objective of this study is to further elucidate biofate of PLGA NPs after intradermal delivery including DCs uptake, and their further trafficking in psoriasis-like mice model by using fluorescence probes. Two-sized DiO/DiI-loaded PLGA NPs of 50 ± 4.9 nm (S-NPs) and 226 ± 7.8 nm (L-NPs) were fabricated, respectively. In vitro cellular uptake results showed that NPs could be internalized into DCs with intact form, and DCs preferred to uptake larger NPs. Consistently, in vivo study showed that L-NPs were more captured by DCs and NPs were firstly transported to skin-draining lymph nodes (SDLN), then to spleens after 8 h injection, whereas more S-NPs were transported into SDLN and spleens. Moreover, FRET imaging showed more structurally intact L-NPs distributed in skins and lymph nodes. In conclusion, particle size can affect the uptake and trafficking of NPs by DCs in skin and lymphoid system, which needs to be considered in NPs tailing to treat inflammatory skin disease like psoriasis.http://www.sciencedirect.com/science/article/pii/S221138352030798XPsoriasisPLGA nanoparticlesFluorescenceDendritic cellsFluorescence resonance energy transferLymphoid organs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zibei Lin Long Xi Shaokui Chen Jinsong Tao Yan Wang Xin Chen Ping Li Zhenping Wang Ying Zheng |
spellingShingle |
Zibei Lin Long Xi Shaokui Chen Jinsong Tao Yan Wang Xin Chen Ping Li Zhenping Wang Ying Zheng Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model Acta Pharmaceutica Sinica B Psoriasis PLGA nanoparticles Fluorescence Dendritic cells Fluorescence resonance energy transfer Lymphoid organs |
author_facet |
Zibei Lin Long Xi Shaokui Chen Jinsong Tao Yan Wang Xin Chen Ping Li Zhenping Wang Ying Zheng |
author_sort |
Zibei Lin |
title |
Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model |
title_short |
Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model |
title_full |
Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model |
title_fullStr |
Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model |
title_full_unstemmed |
Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model |
title_sort |
uptake and trafficking of different sized plga nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model |
publisher |
Elsevier |
series |
Acta Pharmaceutica Sinica B |
issn |
2211-3835 |
publishDate |
2021-04-01 |
description |
Psoriasis is an autoimmune inflammatory disease, where dendritic cells (DCs) play an important role in its pathogenesis. In our previous work, we have demonstrated that topical delivery of curcumin-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) could treat Imiquimod (IMQ)-induced psoriasis-like mice. The objective of this study is to further elucidate biofate of PLGA NPs after intradermal delivery including DCs uptake, and their further trafficking in psoriasis-like mice model by using fluorescence probes. Two-sized DiO/DiI-loaded PLGA NPs of 50 ± 4.9 nm (S-NPs) and 226 ± 7.8 nm (L-NPs) were fabricated, respectively. In vitro cellular uptake results showed that NPs could be internalized into DCs with intact form, and DCs preferred to uptake larger NPs. Consistently, in vivo study showed that L-NPs were more captured by DCs and NPs were firstly transported to skin-draining lymph nodes (SDLN), then to spleens after 8 h injection, whereas more S-NPs were transported into SDLN and spleens. Moreover, FRET imaging showed more structurally intact L-NPs distributed in skins and lymph nodes. In conclusion, particle size can affect the uptake and trafficking of NPs by DCs in skin and lymphoid system, which needs to be considered in NPs tailing to treat inflammatory skin disease like psoriasis. |
topic |
Psoriasis PLGA nanoparticles Fluorescence Dendritic cells Fluorescence resonance energy transfer Lymphoid organs |
url |
http://www.sciencedirect.com/science/article/pii/S221138352030798X |
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