Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines

Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines

Tumor-derived extracellular vesicles (EVs), including exosomes, contain proteins that mirror the molecular landscape of producer cells. Being potentially detectible in biological fluids, EVs are of great interest for the screening of cancer biomarkers. To reveal universal, tissue-specific, and line-...

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Main Authors: Svetlana Novikova, Natalia Shushkova, Tatiana Farafonova, Olga Tikhonova, Roman Kamyshinsky, Victor Zgoda
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:International Journal of Molecular Sciences
Subjects:
extracellular vesicles
exosomes
proteomics
mass-spectrometry
colon cancer
lung cancer
Online Access:https://www.mdpi.com/1422-0067/21/18/6601
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spelling doaj-052a4fb03c864eae89a74ad020c85e692020-11-25T03:47:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-01216601660110.3390/ijms21186601Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell LinesSvetlana Novikova0Natalia Shushkova1Tatiana Farafonova2Olga Tikhonova3Roman Kamyshinsky4Victor Zgoda5Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, 119121 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, 119121 Moscow, RussiaNational Research Center “Kurchatov Institute”, Akademika Kurchatova pl. 1, 123182 Moscow, RussiaOrekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Pogodinskaya 10, 119121 Moscow, RussiaTumor-derived extracellular vesicles (EVs), including exosomes, contain proteins that mirror the molecular landscape of producer cells. Being potentially detectible in biological fluids, EVs are of great interest for the screening of cancer biomarkers. To reveal universal, tissue-specific, and line-specific markers, we performed label-free mass spectrometric profiling of EVs originating from the human colon cancer cell lines Caco-2, HT29, and HCT-116, as well as from the lung cancer cell lines NCI-H23 and A549. A total of 651 proteins was identified in the EV samples using at least two peptides. These proteins were highly enriched in exosome markers. We found 11 universal, eight tissue-specific, and 29 line-specific markers, the levels of which were increased in EVs compared to the whole lysates. The EV proteins were involved in the EGFR, Rap1, integrin, and microRNA signaling associated with metastasis and cancer progression. An EV protein-based assay could be developed as a liquid biopsy tool.https://www.mdpi.com/1422-0067/21/18/6601extracellular vesiclesexosomesproteomicsmass-spectrometrycolon cancerlung cancer
collection DOAJ
language English
format Article
sources DOAJ
author Svetlana Novikova
Natalia Shushkova
Tatiana Farafonova
Olga Tikhonova
Roman Kamyshinsky
Victor Zgoda
spellingShingle Svetlana Novikova
Natalia Shushkova
Tatiana Farafonova
Olga Tikhonova
Roman Kamyshinsky
Victor Zgoda
Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines
International Journal of Molecular Sciences
extracellular vesicles
exosomes
proteomics
mass-spectrometry
colon cancer
lung cancer
author_facet Svetlana Novikova
Natalia Shushkova
Tatiana Farafonova
Olga Tikhonova
Roman Kamyshinsky
Victor Zgoda
author_sort Svetlana Novikova
title Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines
title_short Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines
title_full Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines
title_fullStr Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines
title_full_unstemmed Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines
title_sort proteomic approach for searching for universal, tissue-specific, and line-specific markers of extracellular vesicles in lung and colorectal adenocarcinoma cell lines
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2020-09-01
description Tumor-derived extracellular vesicles (EVs), including exosomes, contain proteins that mirror the molecular landscape of producer cells. Being potentially detectible in biological fluids, EVs are of great interest for the screening of cancer biomarkers. To reveal universal, tissue-specific, and line-specific markers, we performed label-free mass spectrometric profiling of EVs originating from the human colon cancer cell lines Caco-2, HT29, and HCT-116, as well as from the lung cancer cell lines NCI-H23 and A549. A total of 651 proteins was identified in the EV samples using at least two peptides. These proteins were highly enriched in exosome markers. We found 11 universal, eight tissue-specific, and 29 line-specific markers, the levels of which were increased in EVs compared to the whole lysates. The EV proteins were involved in the EGFR, Rap1, integrin, and microRNA signaling associated with metastasis and cancer progression. An EV protein-based assay could be developed as a liquid biopsy tool.
topic extracellular vesicles
exosomes
proteomics
mass-spectrometry
colon cancer
lung cancer
url https://www.mdpi.com/1422-0067/21/18/6601
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AT nataliashushkova proteomicapproachforsearchingforuniversaltissuespecificandlinespecificmarkersofextracellularvesiclesinlungandcolorectaladenocarcinomacelllines
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