DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization

DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization

Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react...

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Bibliographic Details
Main Authors: Claudia Addamiano, Béatrice Gerland, Corinne Payrastre, Jean-Marc Escudier
Format: Article
Language:English
Published: MDPI AG 2016-08-01
Series:Molecules
Subjects:
three way junction
convertible nucleotide
CuACC
chemically modified oligonucleotides
Online Access:http://www.mdpi.com/1420-3049/21/9/1082
Description
Summary:Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 5′-C(S)-functionalization were investigated to evaluate how 3WJ flexibility/stability is affected.
ISSN:1420-3049

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