DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization

DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization

Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react...

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Main Authors: Claudia Addamiano, Béatrice Gerland, Corinne Payrastre, Jean-Marc Escudier
Format: Article
Language:English
Published: MDPI AG 2016-08-01
Series:Molecules
Subjects:
three way junction
convertible nucleotide
CuACC
chemically modified oligonucleotides
Online Access:http://www.mdpi.com/1420-3049/21/9/1082
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spelling doaj-052cab37364e45a380436787d10fb66e2020-11-24T20:40:14ZengMDPI AGMolecules1420-30492016-08-01219108210.3390/molecules21091082molecules21091082DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and CharacterizationClaudia Addamiano0Béatrice Gerland1Corinne Payrastre2Jean-Marc Escudier3Laboratoire de Synthèse et Physico-Chimie de Molécules d′Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, FranceLaboratoire de Synthèse et Physico-Chimie de Molécules d′Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, FranceLaboratoire de Synthèse et Physico-Chimie de Molécules d′Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, FranceLaboratoire de Synthèse et Physico-Chimie de Molécules d′Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, FranceConstruction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 5′-C(S)-functionalization were investigated to evaluate how 3WJ flexibility/stability is affected.http://www.mdpi.com/1420-3049/21/9/1082three way junctionconvertible nucleotideCuACCchemically modified oligonucleotides
collection DOAJ
language English
format Article
sources DOAJ
author Claudia Addamiano
Béatrice Gerland
Corinne Payrastre
Jean-Marc Escudier
spellingShingle Claudia Addamiano
Béatrice Gerland
Corinne Payrastre
Jean-Marc Escudier
DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
Molecules
three way junction
convertible nucleotide
CuACC
chemically modified oligonucleotides
author_facet Claudia Addamiano
Béatrice Gerland
Corinne Payrastre
Jean-Marc Escudier
author_sort Claudia Addamiano
title DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
title_short DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
title_full DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
title_fullStr DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
title_full_unstemmed DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization
title_sort dna three way junction core decorated with amino acids-like residues-synthesis and characterization
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2016-08-01
description Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 5′-C(S)-functionalization were investigated to evaluate how 3WJ flexibility/stability is affected.
topic three way junction
convertible nucleotide
CuACC
chemically modified oligonucleotides
url http://www.mdpi.com/1420-3049/21/9/1082
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